Portugal J, Waring M J
Eur J Biochem. 1987 Sep 1;167(2):281-9. doi: 10.1111/j.1432-1033.1987.tb13334.x.
Techniques of DNase I and micrococcal nuclease footprinting have been used to compare the binding sites for berenil, netropsin and distamycin on two different DNA fragments. Each ligand binds to the A + T-rich zones which contain clusters of at least four A.T base pairs. Neither guanosine nor cytidine nucleotides appear to be allowed within the A + T-rich runs which constitute the preferred binding sites, although they are sometimes protected from DNase I cleavage in neighbouring regions. Berenil and netropsin share with distamycin the property of causing enhanced rates of cleavage at certain sequences flanking their binding sites. There are significant differences in the concentrations of each ligand required to produce defined patterns of protection, seemingly dependent upon the nature (and possibly the gross base composition) of the piece of DNA being used in the experiment.
已运用脱氧核糖核酸酶I和微球菌核酸酶足迹法技术，比较了贝尼尔、纺锤菌素和偏端霉素在两条不同DNA片段上的结合位点。每种配体都与富含A+T的区域结合，这些区域包含至少四个A·T碱基对的簇。构成优先结合位点的富含A+T的序列中似乎不允许鸟苷酸或胞苷酸存在，尽管它们有时在相邻区域可免受脱氧核糖核酸酶I的切割。贝尼尔和纺锤菌素与偏端霉素具有共同特性，即在其结合位点侧翼的某些序列处会导致切割速率加快。产生特定保护模式所需的每种配体浓度存在显著差异，这似乎取决于实验中所用DNA片段的性质（可能还有总体碱基组成）。
