高通量测序鉴定 EV71 和 CA16 感染恒河猴外周血单个核细胞后新型 microRNA。

High-Throughput Sequencing of Putative Novel microRNAs in Rhesus Monkey Peripheral Blood Mononuclear Cells following EV71 and CA16 Infection.

机构信息

Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming, China.

出版信息

Intervirology. 2018;61(3):133-142. doi: 10.1159/000493798. Epub 2018 Nov 7.

DOI:10.1159/000493798

PMID:30404089

Abstract

OBJECTIVES

Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) remain the major pathogens in hand, foot, and mouth disease (HFMD) cases, but the mechanisms of the different pathogeneses that follow EV71 and CA16 infection remain largely unknown.

METHODS

Herein, we utilized microRNA (miRNA) deep sequencing to investigate the roles of novel differentially expressed miRNAs in peripheral blood mononuclear cells (PBMCs) infected with EV71 and CA16.

RESULTS

The results identified 13 novel differentially expressed miRNAs in each group. Additionally, the target genes were predicted by the miRanda and RNAhybrid programs, and a total of 2,501 targets were found in the two databases. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that these targets were mainly involved in cell development and were associated with nervous system development, system development, multicellular organism development, the Wnt signaling pathway, the PDGF signaling pathway, and the EGF receptor signaling pathway. Finally, a coexpression regulatory network was built with the key targets to further extrapolate the functional interactions of the targets and their coexpressed genes.

CONCLUSION

Our results not only revealed potential biomarkers or targets for the diagnosis and treatment of HFMD, but also provided new insights to explore the mechanisms of EV71 and CA16 pathogenesis.

摘要

目的

肠道病毒 71 型（EV71）和柯萨奇病毒 A16 型（CA16）仍是手足口病（HFMD）病例的主要病原体，但 EV71 和 CA16 感染后不同发病机制的机制在很大程度上仍不清楚。

方法

本研究利用 microRNA（miRNA）深度测序来探讨新型差异表达 miRNA 在 EV71 和 CA16 感染外周血单个核细胞（PBMCs）中的作用。

结果

结果在每组中鉴定出 13 种新型差异表达 miRNA。此外，通过 miRanda 和 RNAhybrid 程序预测了靶基因，在两个数据库中共发现了 2501 个靶基因。然后，基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析表明，这些靶基因主要参与细胞发育，并与神经系统发育、系统发育、多细胞生物发育、Wnt 信号通路、PDGF 信号通路和 EGF 受体信号通路有关。最后，构建了一个关键靶基因的共表达调控网络，以进一步推断靶基因及其共表达基因的功能相互作用。