Department of Infectious, Respiratory and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
Int J Mol Sci. 2018 Nov 6;19(11):3497. doi: 10.3390/ijms19113497.
Adipose-derived mesenchymal stem cells (ADSCs) have become a common cell source for cell transplantation therapy. Clinical studies have used ADSCs to develop treatments for tissue fibrosis, such as liver cirrhosis and pulmonary fibroma. The need to examine and compare basic research data using clinical research data derived from mice and humans is expected to increase in the future. Here, to better characterize the cells, the protein components expressed by human ADSCs used for treatment, and mouse ADSCs used for research, were comprehensively analyzed by liquid chromatography with tandem mass spectrometry. We found that 92% (401 type proteins) of the proteins expressed by ADSCs in humans and mice were consistent. When classified by the protein functions in a gene ontology analysis, the items that differed by >5% between human and mouse ADSCs were "biological adhesion, locomotion" in biological processes, "plasma membrane" in cellular components, and "antioxidant activity, molecular transducer activity" in molecular functions. Most of the listed proteins were sensitive to cell isolation processes. These results show that the proteins expressed by human and murine ADSCs showed a high degree of correlation.
脂肪间充质干细胞（ADSCs）已成为细胞移植治疗的常用细胞来源。临床研究已经使用 ADSCs 开发了治疗组织纤维化的方法，如肝硬化和肺纤维瘤。未来，预计将需要使用源自小鼠和人类的临床研究数据来检查和比较基础研究数据。在这里，为了更好地描述细胞，通过液相色谱-串联质谱法全面分析了用于治疗的人 ADSCs 和用于研究的小鼠 ADSCs 表达的蛋白质成分。我们发现，人类和小鼠 ADSCs 中表达的蛋白质有 92%（401 种蛋白质）是一致的。在基因本体分析中按蛋白质功能分类时，人类和小鼠 ADSCs 之间差异>5%的项目是生物过程中的“生物粘附，运动”，细胞成分中的“质膜”，以及分子功能中的“抗氧化活性，分子转导活性”。列出的大多数蛋白质对细胞分离过程敏感。这些结果表明，人和鼠 ADSCs 表达的蛋白质具有高度相关性。
