Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
Int J Mol Sci. 2019 May 30;20(11):2672. doi: 10.3390/ijms20112672.
Although cell therapy using adipose-derived mesenchymal stem cells (AdMSCs) regulates immunity, the degree to which cell quality and function are affected by differences in immunodeficiency of donors is unknown. We used liquid chromatography tandem-mass spectrometry (LC MS/MS) to identify the proteins expressed by mouse AdMSCs (mAsMSCs) isolated from normal (C57BL/6) mice and mice with severe combined immunodeficiency (SCID). The protein expression profiles of each strain were 98%-100% identical, indicating that the expression levels of major proteins potentially associated with the therapeutic effects of mAdMSCs were highly similar. Further, comparable levels of cell surface markers (CD44, CD90.2) were detected using flow cytometry or LC MS/MS. MYH9, ACTN1, CANX, GPI, TPM1, EPRS, ITGB1, ANXA3, CNN2, MAPK1, PSME2, CTPS1, OTUB1, PSMB6, HMGB1, RPS19, SEC61A1, CTNNB1, GLO1, RPL22, PSMA2, SYNCRIP, PRDX3, SAMHD1, TCAF2, MAPK3, RPS24, and MYO1E, which are associated with immunity, were expressed at higher levels by the SCID mAdMSCs compared with the C57BL/6 mAdMSCs. In contrast, ANXA9, PCBP2, LGALS3, PPP1R14B, and PSMA6, which are also associated with immunity, were more highly expressed by C57BL/6 mAdMSCs than SCID mAdMSCs. These findings implicate these two sets of proteins in the pathogenesis and maintenance of immunodeficiency.
虽然脂肪来源间充质干细胞（AdMSCs）的细胞治疗可调节免疫，但供者免疫缺陷差异对细胞质量和功能的影响程度尚不清楚。我们使用液相色谱-串联质谱（LC-MS/MS）鉴定了从小鼠（C57BL/6）和严重联合免疫缺陷（SCID）小鼠分离的小鼠 AdMSCs（mAsMSCs）中表达的蛋白质。两种品系的蛋白质表达谱的相似度为 98%-100%，表明与 mAdMSCs 治疗效果相关的主要蛋白的表达水平高度相似。此外，通过流式细胞术或 LC-MS/MS 检测到细胞表面标志物（CD44、CD90.2）的水平相当。MYH9、ACTN1、CANX、GPI、TPM1、EPRS、ITGB1、ANXA3、CNN2、MAPK1、PSME2、CTPS1、OTUB1、PSMB6、HMGB1、RPS19、SEC61A1、CTNNB1、GLO1、RPL22、PSMA2、SYNCRIP、PRDX3、SAMHD1、TCAF2、MAPK3、RPS24 和 MYO1E 等与免疫相关的蛋白在 SCID mAdMSCs 中的表达水平高于 C57BL/6 mAdMSCs。相比之下，ANXA9、PCBP2、LGALS3、PPP1R14B 和 PSMA6 等也与免疫相关的蛋白在 C57BL/6 mAdMSCs 中的表达水平高于 SCID mAdMSCs。这些发现提示这两组蛋白与免疫缺陷的发病机制和维持有关。
