Xie Yu, Zhou Yunlei, Xi Wenming, Zeng Feng, Chen Songyue
School of Aerospace Engineering, Xiamen University, Xiamen 361005, China.
Micromachines (Basel). 2016 Aug 4;7(8):131. doi: 10.3390/mi7080131.
Automation of cell microinjection greatly reduces operational difficulty, but cell fixation remains a challenge. Here, we describe an innovative device that solves the fixation problem without single-cell operation. The microarray cylinder is designed with a polydimethylsiloxane (PDMS) material surface to control the contact force between cells and the material. Data show that when the injection velocity exceeds 1.5 mm/s, microinjection success rate is over 80%. The maximum value of the adhesion force between the PDMS plate and the cell is 0.0138 N, and the need can be met in practical use of the robotic microinjection.
细胞显微注射的自动化极大地降低了操作难度，但细胞固定仍然是一个挑战。在此，我们描述了一种创新装置，该装置无需单细胞操作即可解决固定问题。微阵列圆柱体采用聚二甲基硅氧烷（PDMS）材料表面设计，以控制细胞与材料之间的接触力。数据显示，当注射速度超过1.5毫米/秒时，显微注射成功率超过80%。PDMS板与细胞之间的粘附力最大值为0.0138牛，在机器人显微注射的实际应用中能够满足需求。
