Clementi M, Testa I, Festa A, Bagnarelli P, Chang C M, Carloni G
FEBS Lett. 1987 Aug 31;221(1):11-7. doi: 10.1016/0014-5793(87)80343-5.
Several human cell lines derived from primary cancer of the liver are able to grow under serum-free conditions and produce spreading and growth factors which are released into the culture medium. Since this autocrine growth under hormone-free conditions might play a basic role in malignant transformation, we studied the effect on cell replication and the presence of specific membrane receptors of epidermal growth factor (EGF) and insulin on a dedifferentiated human hepatoma cell line, named HA22T/VGH. Our results point to a similar inhibitory effect on cell replication in the presence of both EGF and insulin, in spite of detecting different affinities of binding.
几种源自原发性肝癌的人类细胞系能够在无血清条件下生长，并产生释放到培养基中的扩散和生长因子。由于这种在无激素条件下的自分泌生长可能在恶性转化中起基本作用，我们研究了表皮生长因子（EGF）和胰岛素对一种去分化的人类肝癌细胞系（名为HA22T/VGH）的细胞复制的影响以及特定膜受体的存在情况。尽管检测到不同的结合亲和力，但我们的结果表明，在同时存在EGF和胰岛素的情况下，对细胞复制有类似的抑制作用。
