Clementi M, Festa A, Testa I, Bagnarelli P, Devescovi G, Carloni G
Institute of Microbiology of Trieste, Italy.
FEBS Lett. 1989 Jun 5;249(2):297-301. doi: 10.1016/0014-5793(89)80645-3.
Data are presented from a comparative research on expression of epidermal growth factor (EGF) receptors and response to EGF of six independently established cell lines derived from human hepatoma. These lines differ in terms of the degree of differentiation, presence of hepatitis B virus (HBV) DNA copies in integrated form and expression of HBV genes. Our results indicate differential expression of membrane EGF receptors and differential response to EGF under serum- and hormone-free culture conditions. Furthermore, a significant difference in affinity could be detected between EGF receptors of the two highly dedifferentiated cell lines (HA22T/VGH and Li7A) whose replication is inhibited by EGF concentrations capable of stimulating more differentiated phenotypes.
本文展示了一项关于六种独立建立的人肝癌细胞系中表皮生长因子（EGF）受体表达及对EGF反应的比较研究数据。这些细胞系在分化程度、整合形式的乙型肝炎病毒（HBV）DNA拷贝数以及HBV基因表达方面存在差异。我们的结果表明，在无血清和无激素培养条件下，膜EGF受体存在差异表达，对EGF的反应也有所不同。此外，在两种高度去分化细胞系（HA22T/VGH和Li7A）的EGF受体之间可检测到亲和力的显著差异，其复制受到能够刺激更分化表型的EGF浓度的抑制。
