Fichna P, Malendowicz L K
Folia Histochem Cytobiol. 1987;25(1):3-15.
The critical evaluation of isolation methods for obtaining the adrenocortical cell suspension due to trypsin or collagenase digestion was done. Some collagenase advantages were indicated by morphological observations on the staining smears as well as by ACTH stimulation test. The cytochemical reactions for enzyme activities had the limited applications for those purposes. It also appeared that commonly applied dye exclusion tests were inadequate for characterization of cell suspension. The possible role of the adrenocortical cell debris in the basal corticosterone production was pointed out. The maintenance of the sex dimorphism and the functional differences in the adrenocortical cells isolated from male and female rats have been observed.
对通过胰蛋白酶或胶原酶消化获取肾上腺皮质细胞悬液的分离方法进行了批判性评估。通过染色涂片的形态学观察以及促肾上腺皮质激素刺激试验表明了胶原酶的一些优势。酶活性的细胞化学反应在这些目的上应用有限。还发现常用的染料排除试验不足以表征细胞悬液。指出了肾上腺皮质细胞碎片在基础皮质酮产生中的可能作用。观察到从雄性和雌性大鼠分离的肾上腺皮质细胞中性别二态性和功能差异的维持情况。
