Saidi Rakia, Ghrab Ferdaws, Kallel Rim, Feki Abdelfattah El, Boudawara Tahya, Chesné Christophe, Ammar Emna, Jarraya Raoudha Mezghani
Laboratory of Organic Chemistry LR17ES08, Natural Substances team, Faculty of Sciences of Sfax, Sfax University.
Coastal and urban environments, National Engineering School of Sfax, Sfax University.
J Oleo Sci. 2018;67(11):1483-1499. doi: 10.5650/jos.ess18056.
The aerial part of Clematis flammula (Ranunculaceae) has been traditionally used in the treatment of skin diseases including mycotic infection in the Tunisian traditional medicine. The study was undertaken to extract and determine the essential oil chemical composition of Clematis flammula aerial parts and to assess the potential of anemonin in wound healing on mechanically wounded wistar rats. The essential oil was obtained by hydrodistillation and analyzed by GC-MS. Anemonin was isolated and then incorporated as active in a cream for which the cytotoxicity was evaluated by methyl thiazolyl tetrazolium (MTT)-based colorimetric assay. Then, its potential in wound healing on mechanically wounded wistar rats was assessed. The GC-MS analysis showed that the major compound was protoanemonin (86.74%) which spontaneously dimerised in part to form the anemonin. The wound healing activity of anemonin cream exhibited a non toxic potential of anemonin at a concentration of 25 µg/mL with a cell migration efficiency that reaches more than 80% after 48 hours of treatment. Wound healing efficiency was evaluated by monitoring morphological and skin histological analyses. Comparable wound surface reduction of the group treated by anemonin cream (p ≥ 0.05) when compared to the reference treated group. The skin histological analysis showed the completely wound closure. Antioxidant activity was assessed by the malondialdehyde (MDA) rates and antioxidant enzymes (glutathione peroxidase (GPx) and catalase) determination. The results provided strong support for the effective wound healing activity of anemonin cream, making it a promising candidate as a therapeutic agent in tissue repairing processes.
在突尼斯传统医学中，毛茛科植物小毛茛的地上部分传统上用于治疗包括真菌感染在内的皮肤病。本研究旨在提取并测定小毛茛地上部分的精油化学成分，并评估白头翁素对机械性损伤的Wistar大鼠伤口愈合的潜在作用。通过水蒸馏法获得精油，并采用气相色谱 - 质谱联用（GC - MS）进行分析。分离出白头翁素，然后将其作为活性成分掺入乳膏中，通过基于甲基噻唑基四氮唑（MTT）的比色法评估该乳膏的细胞毒性。然后，评估其对机械性损伤的Wistar大鼠伤口愈合的潜在作用。GC - MS分析表明，主要化合物是原白头翁素（86.74%），其部分自发二聚形成白头翁素。白头翁素乳膏的伤口愈合活性表明，在浓度为25μg/mL时，白头翁素具有无毒潜力，处理48小时后细胞迁移效率达到80%以上。通过监测形态学和皮肤组织学分析评估伤口愈合效率。与参考治疗组相比，白头翁素乳膏治疗组的伤口表面减少情况相当（p≥0.05）。皮肤组织学分析显示伤口完全愈合。通过丙二醛（MDA）含量以及抗氧化酶（谷胱甘肽过氧化物酶（GPx）和过氧化氢酶）的测定评估抗氧化活性。结果为白头翁素乳膏有效的伤口愈合活性提供了有力支持，使其成为组织修复过程中有前景的治疗剂候选物。
