Ning Yu-Ming, Rao Yue-Feng, Liang Wen-Quan
School of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(5):393-6.
To investigate the effect of different permeation enhancer on transdermal permeation of anemonin through human skin.
The permeation experiments were performed using human skin on modified Franz diffusion cells in vitro. The concentrations of anemonin in receptor compartment at specified time points were determined by HPLC. The steady flux and the cumulative quantity of anemonin through skin were calculated.
The flux of anemonin permeating through human skin from 30% ethanol, 50% ethanol solution and a combination of 3% laurocapm -5% polysorbate 20 and 30% ethanol -3 % laurocapm -5% polysorbate 20 of anemonin was (9.30 +/- 0.32), (18.56+/-0.58), (7.29+/-0.35), (13.77+/-0. 16) microg x cm(-2) x h(-1) and 7.9, 15.9, 6.2, 11.8 times higher than from saturated water solution respectively.
Ethanol and laurocapm can remarkably improve the transdermal permeation of anemonin and the anemonin have the potential to be developed to new transdermal preparation.
研究不同渗透促进剂对白头翁素经人皮渗透的影响。
采用改良的Franz扩散池，以人皮进行体外渗透实验。在特定时间点，通过高效液相色谱法测定接受池中白头翁素的浓度。计算白头翁素透过皮肤的稳态通量和累积量。
白头翁素在30%乙醇、50%乙醇溶液以及3%月桂氮卓酮-5%聚山梨酯20与30%乙醇-3%月桂氮卓酮-5%聚山梨酯20组合中的透皮通量分别为(9.30±0.32)、(18.56±0.58)、(7.29±0.35)、(13.77±0.16)μg·cm⁻²·h⁻¹，分别比其在饱和水溶液中的透皮通量高7.9、15.9、6.2、11.8倍。
乙醇和月桂氮卓酮可显著提高白头翁素的透皮渗透，白头翁素有开发成新型透皮制剂的潜力。
