生长抑素及其受体1 - 5在子宫内膜异位症组织和细胞中的表达。
Expression of somatostatin and its receptor 1-5 in endometriotic tissues and cells.
作者信息
Zhao Yanhua, Peng Lin, Li Xiang, Zhang Yi
机构信息
Department of Obstetrics and Gynecology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
Reproductive and Stem Cell Research Institute, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
出版信息
Exp Ther Med. 2018 Nov;16(5):3777-3784. doi: 10.3892/etm.2018.6730. Epub 2018 Sep 12.
The present study aimed to detect the expression of somatostatin (SS) and SS receptor (SSTR)1-5 in tissues from patients with endometriosis (EMS). Reverse transcription-quantitative polymerase chain reaction analysis was applied to examine the expression of somatostatin gene in ectopic endometrial cells (EECs). The expression of somatostatin receptor 1-5 in the ectopic endometrium (EE), eutopic endometrium and normal endometrium and their association with EMS staging were determined by immunohistochemistry. The results indicated that the expression of SS in EECs was significantly higher compared with that in the control group. SSTR1-5 were expressed in the EE tissues from 30 patients with EMS, and the positive rates were 43.3, 70.0, 53.3, 50.0 and 96.7%, respectively, which were closely associated with EMS staging of the patients. The positive rates of SSTR1-5 expression in the eutopic endometrium from 12 patients with EMS were 33.3, 41.7, 58.3, 58.3 and 83.3%, respectively, while the positive rates of SSTR1-5 expression in the normal endometrium from 14 women without EMS were 7.1, 7.1, 21.4, 28.6 and 64.3%, which were lower than the positive rates of SSTR1-5 in the EE (43.3, 70, 53.3, 50 and 96.7%) and eutopic endometrial cells (33.3, 41.7, 58.3, 58.3 and 83.3%). In conclusion, SS was highly expressed in EECs. SSTR1-5 were expressed in the ectopic as well as eutopic endometrium, and low or moderate expression of SSTR1-4 and high expression of SSTR5 were detected in the ectopic and eutopic endometrial tissues, while low expression of SSTR1-4 and partial expression of SSTR5 were detected in normal endometrium. The positive rates of expression of SSTR1-5 in the EE cells and eutopic endometrium were higher than those in the normal endometrium. The expression of all the subtypes of SSTR in the EE tissues was closely associated with EMS staging.
本研究旨在检测子宫内膜异位症（EMS）患者组织中生长抑素（SS）及生长抑素受体（SSTR）1 - 5的表达。应用逆转录 - 定量聚合酶链反应分析检测异位子宫内膜细胞（EECs）中生长抑素基因的表达。采用免疫组织化学法测定异位内膜（EE）、在位内膜及正常内膜中生长抑素受体1 - 5的表达及其与EMS分期的关系。结果表明，EECs中SS的表达显著高于对照组。30例EMS患者的EE组织中均有SSTR1 - 5表达，阳性率分别为43.3%、70.0%、53.3%、50.0%和96.7%，且与患者的EMS分期密切相关。12例EMS患者在位内膜中SSTR1 - 5表达的阳性率分别为33.3%、41.7%、58.3%、58.3%和83.3%，而14例无EMS的女性正常内膜中SSTR1 - 5表达的阳性率分别为7.1%、7.1%、21.4%、28.6%和64.3%，低于EE中SSTR1 - 5的阳性率（43.3%、70%、53.3%、50%和96.7%）以及在位子宫内膜细胞中SSTR1 - 5的阳性率（33.3%、41.7%、58.3%、58.3%和83.3%）。综上所述，SS在EECs中高表达。SSTR1 - 5在异位及在位内膜中均有表达，异位和在位内膜组织中检测到SSTR1 - 4低或中度表达、SSTR5高表达，而正常内膜中检测到SSTR1 - 4低表达及SSTR5部分表达。EE细胞和在位内膜中SSTR1 - 5的表达阳性率高于正常内膜。EE组织中所有SSTR亚型的表达均与EMS分期密切相关。
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