Dong Xuetao, Liu Yandi
Department of Gastroenterology, Tianjin Union Medical Centre, Tianjin 300120, P.R. China.
Oncol Lett. 2018 Nov;16(5):5769-5774. doi: 10.3892/ol.2018.9324. Epub 2018 Aug 17.
Clinical significance of micro ribonucleic acid (miR)-24 and miR-101 were investigated by evaluating the expression of miR-24 and miR-101 in the tissues of patients with advanced gastric cancer. A total of 247 gastric cancer tissue specimens and 150 cancer-adjacent normal tissues (>5 cm away from the tumor) from patients with advanced gastric cancer who underwent surgical resection in the Surgical Oncology Department of Tianjin Union Medical Centre (Tianjin, China) from April 2013 to May 2016 were collected. The expression of miR-24 and miR-101 in gastric cancer and cancer-adjacent normal tissues were detected via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and the correlation of the levels of miR-24 and miR-101 in gastric cancer tissues with their clinical and pathological features were explored. The expression level of miR-24 in gastric cancer tissues was significantly higher than that in cancer-adjacent normal tissues (t=10.26, p<0.01), while the expression level of miR-101 was significantly lower (t=13.940, p<0.01). The expression of miR-24 and miR-101 in gastric cancer was correlated with the pathological differentiation degree of the tumor, lymph node metastasis and depth of infiltration (p<0.05). The multivariate Cox regression analysis revealed that miR-24 and miR-101 were independent prognostic factors affecting the overall survival of patients (p<0.01). The results indicated that the expression of miR-24 is upregulated and that of miR-101 is downregulated in gastric cancer tissues. miR-24 and miR-101 may promote the occurrence, development, infiltration and metastasis of gastric cancer, and can be indicators for the prognosis of patients with gastric cancer.
通过评估晚期胃癌患者组织中微小核糖核酸（miR）-24和miR-101的表达，研究其临床意义。收集了2013年4月至2016年5月在天津医科大学肿瘤医院（中国天津）外科肿瘤科接受手术切除的晚期胃癌患者的247份胃癌组织标本和150份癌旁正常组织标本（距肿瘤>5 cm）。通过逆转录-定量聚合酶链反应（RT-qPCR）检测胃癌组织和癌旁正常组织中miR-24和miR-101的表达，并探讨胃癌组织中miR-24和miR-101水平与其临床病理特征的相关性。胃癌组织中miR-24的表达水平显著高于癌旁正常组织（t=10.26，p<0.01），而miR-101的表达水平显著低于癌旁正常组织（t=13.940，p<0.01）。胃癌中miR-24和miR-101的表达与肿瘤的病理分化程度、淋巴结转移及浸润深度相关（p<0.05）。多因素Cox回归分析显示，miR-24和miR-101是影响患者总生存的独立预后因素（p<0.01）。结果表明，胃癌组织中miR-24表达上调，miR-101表达下调。miR-24和miR-101可能促进胃癌的发生、发展、浸润和转移，可作为胃癌患者预后的指标。
