Department of Inspection, Weifang People's Hospital, Weifang, Shandong, China (mainland).
Department of Breast Surgery, Weifang People's Hospital, Weifang, Shandong, China (mainland).
Med Sci Monit. 2018 Nov 7;24:7988-7995. doi: 10.12659/MSM.910809.
BACKGROUND The aim of this study was to explore the expression levels of micro ribonucleic acid-10b (miR-10b) and micro ribonucleic acid-181b (miR-181b) in gastric cancer tissues, as well as their application value in clinical diagnosis and treatment. MATERIAL AND METHODS A total of 120 patients with gastric cancer who were diagnosed and treated in the Department of Gastroenterology of our hospital were enrolled in this study. The gastric cancer tissues and paracancerous tissues were collected for measuring the expression of miR-10b and miR-181b by in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR). The 5-year survival rate was also analyzed. RESULTS The expressions of miR-10b and miR-181b in gastric cancer tissues were both upregulated and were significantly higher than those in the paracancerous tissues (p<0.05). In addition, the expressions of miR-10b and miR-181b in gastric cancer tissues were correlated with tumor size, degree of pathological differentiation, depth of infiltration, tumor node metastasis (TNM) staging, and lymph node metastasis, as well as local lymph node and distant metastasis (p<0.05). For patients in stage II and III, the expressions of miR-10b and miR-181b were significantly correlated with the 5-year survival rate. CONCLUSIONS The high expressions of miR-10b and miR-181b are significantly correlated with poor prognosis in stage II and III patients with gastric cancer, suggesting that their expressions might be criteria for evaluating the prognosis of gastric cancer.
本研究旨在探讨微小核糖核酸-10b（miR-10b）和微小核糖核酸-181b（miR-181b）在胃癌组织中的表达水平及其在临床诊断和治疗中的应用价值。
本研究共纳入 120 例在我院消化内科诊断和治疗的胃癌患者，收集胃癌组织和癌旁组织，采用原位杂交和逆转录-聚合酶链反应（RT-PCR）检测 miR-10b 和 miR-181b 的表达，并分析其 5 年生存率。
胃癌组织中 miR-10b 和 miR-181b 的表达均上调，明显高于癌旁组织（p<0.05）。此外，胃癌组织中 miR-10b 和 miR-181b 的表达与肿瘤大小、病理分化程度、浸润深度、肿瘤淋巴结转移（TNM）分期以及淋巴结转移、局部淋巴结和远处转移有关（p<0.05）。对于 II 期和 III 期患者，miR-10b 和 miR-181b 的表达与 5 年生存率显著相关。
miR-10b 和 miR-181b 的高表达与 II 期和 III 期胃癌患者的预后不良显著相关，提示其表达可能是评估胃癌预后的标准。
