Zeng Yuan, Xiao Dakai, He Huiming, He Jiaxi, Pan Hui, Yang Wei, Chen Yaoqi, He Jianxing
Department of Thoracic Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China.
State Key Laboratory of Respiratory Diseases and National Clinical Research Center for Respiratory Disease and Guangzhou Institute of Respiratory Diseases, Guangzhou, Guangdong 510000, P.R. China.
Oncol Lett. 2018 Nov;16(5):5916-5922. doi: 10.3892/ol.2018.9403. Epub 2018 Sep 5.
Serine incorporator 2 (SERINC2) is a member of the SERINC family of transmembrane proteins that incorporate serine into membrane lipids during synthesis. In the present study, the biological function of SERINC2 in lung adenocarcinoma cells was investigated. The data from a previous study and the publicly available Oncomine database were analysed regarding the expression levels of SERINC2 mRNA in lung adenocarcinoma. A lentiviral-based short hairpin RNA (shRNA) was used to suppress SERINC2 expression in lung cancer cells. The effect of SERINC2 expression on lung cancer proliferation was determined using cell counting kit-8 and colony formation assays. The influence on invasion and migration was examined using Transwell and wound-healing assays, respectively. Phosphorylated protein kinase B (p-AKT) expression levels were assessed by immunoblotting. According to a previous study and Oncomine, expression levels of SERINC2 mRNA are significantly upregulated in tumour tissues compared with those in healthy tissues in patients with lung adenocarcinoma. SERINC2-knockdown by lentiviral-based shRNA inhibited the proliferation, migration and invasion of the H1650 and A549 cells. In addition, p-AKT expression levels were significantly decreased following SERINC2-knockdown. In conclusion, SERINC2-knockdown suppresses lung adenocarcinoma proliferation, migration and invasion through a mechanism that may be associated with phosphatidylinositol 3-kinase/AKT signalling. Based on these findings, SERINC2 serves an important role in the progression of lung adenocarcinoma.
丝氨酸整合蛋白2（SERINC2）是跨膜蛋白SERINC家族的成员之一，在合成过程中将丝氨酸整合到膜脂中。在本研究中，对SERINC2在肺腺癌细胞中的生物学功能进行了研究。分析了先前研究的数据以及公开可用的Oncomine数据库中关于肺腺癌中SERINC2 mRNA的表达水平。使用基于慢病毒的短发夹RNA（shRNA）来抑制肺癌细胞中SERINC2的表达。使用细胞计数试剂盒-8和集落形成试验确定SERINC2表达对肺癌增殖的影响。分别使用Transwell试验和伤口愈合试验检测对侵袭和迁移的影响。通过免疫印迹评估磷酸化蛋白激酶B（p-AKT）的表达水平。根据先前的研究和Oncomine数据库，与肺腺癌患者的健康组织相比，肿瘤组织中SERINC2 mRNA的表达水平显著上调。基于慢病毒的shRNA敲低SERINC2可抑制H1650和A549细胞的增殖、迁移和侵袭。此外，敲低SERINC2后p-AKT表达水平显著降低。总之，敲低SERINC2可通过一种可能与磷脂酰肌醇3-激酶/AKT信号传导相关的机制抑制肺腺癌的增殖、迁移和侵袭。基于这些发现，SERINC2在肺腺癌进展中起重要作用。
