Zhou Jixiang, Li Xiaoli
Department of Hepatobiliary Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Oncol Lett. 2018 Nov;16(5):6633-6637. doi: 10.3892/ol.2018.9458. Epub 2018 Sep 19.
This study investigated the effects of phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression on liver function classification, serum tumor markers and liver function of patients with liver cancer. A total of 63 patients with primary liver cancer treated in Xiangya Hospital (Changsha, China) were retrospectively analyzed. The difference in the PTEN expression levels in normal liver cells and liver cancer cells was compared via immunohistochemistry. According to the expression level of PTEN in the patient's pathological report, patients were divided into PTEN-positive and PTEN-negative groups. The expression level of each tumor marker in serum of patients was observed, and the association of PTEN expression level with the serum tumor markers was analyzed. Moreover, the changes in liver function and inflammatory factors before and after chemotherapy were compared. Finally, the relationship between the PTEN expression level and Child-Pugh grading of the liver function was detected. Compared with that in normal liver cells, the positive expression rate of PTEN protein in liver cancer cells was significantly decreased (P<0.05). No significant difference was found in the expression levels of serum tumor markers, except α-fetoprotein (AFP) in liver cancer patients between PTEN-positive and PTEN-negative group, indicating that PTEN expression has no significant effects on serum tumor markers. The levels of albumin (ALB), alkaline phosphatase (ALP) and prothrombin activity (PTA) were decreased significantly after chemotherapy compared with those before chemotherapy (P<0.05). Besides, the levels of inflammatory factors were remarkably reduced after chemotherapy. PTEN expression was negatively associated with liver function grading, and the higher the PTEN expression, the lower the liver function grading was. The low expression of PTEN has a certain association with the occurrence and grading of liver cancer. PTEN gene has guiding significance in predicting the occurrence, development and prognosis of liver cancer.
本研究探讨了第10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）表达对肝癌患者肝功能分级、血清肿瘤标志物及肝功能的影响。回顾性分析了在湘雅医院（中国长沙）接受治疗的63例原发性肝癌患者。通过免疫组织化学比较正常肝细胞和肝癌细胞中PTEN表达水平的差异。根据患者病理报告中PTEN的表达水平，将患者分为PTEN阳性组和PTEN阴性组。观察患者血清中各肿瘤标志物的表达水平，并分析PTEN表达水平与血清肿瘤标志物的相关性。此外，比较化疗前后肝功能和炎症因子的变化。最后，检测PTEN表达水平与肝功能Child-Pugh分级的关系。与正常肝细胞相比，肝癌细胞中PTEN蛋白的阳性表达率显著降低（P<0.05）。PTEN阳性组和PTEN阴性组肝癌患者血清肿瘤标志物表达水平除甲胎蛋白（AFP）外，差异无统计学意义，表明PTEN表达对血清肿瘤标志物无显著影响。化疗后白蛋白（ALB）、碱性磷酸酶（ALP）和凝血酶原活动度（PTA）水平较化疗前显著降低（P<0.05）。此外，化疗后炎症因子水平显著降低。PTEN表达与肝功能分级呈负相关，PTEN表达越高，肝功能分级越低。PTEN低表达与肝癌的发生及分级有一定关联。PTEN基因对预测肝癌的发生、发展及预后具有指导意义。
