Yagawa K, Hayashi S, Nakanishi M, Aso H, Ogata K, Maruyama M, Ichinose Y, Shigematsu N
Int J Immunopharmacol. 1987;9(4):497-501. doi: 10.1016/0192-0561(87)90025-7.
The superoxide (O2-) generating activity of human polymorphonuclear leukocytes (PMN) was suppressed more than 50% compared with control cells by preincubation of the cells with soluble immune complexes (Fc receptor-mediated desensitization). The suppression was observed when wheat germ agglutinin (WGA) or N-formyl-L-methionyl-L-leucyl-phenylalanine (fMLP) was used as stimuli for O2- generation. However, the desensitized cells could produce as much O2- as control cells when they were stimulated with phorbol myristate acetate (PMA). The desensitized cells were pre-exposed to a small dose of PMA for a short period (2-3 min). By this procedure, the activity of the cells for WGA or fMLP induced O2- generation was almost completely restored. Similarly, the inhibitory effect of 3'-deazaadenosing on WGA, fMLP or immune complexes (IC) induced O2- generation was abolished by the short-term pre-exposure of the cells to a small dose of PMA.
通过用可溶性免疫复合物对人多形核白细胞（PMN）进行预孵育（Fc受体介导的脱敏），与对照细胞相比，这些细胞产生超氧化物（O2-）的活性被抑制了50%以上。当用小麦胚凝集素（WGA）或N-甲酰-L-蛋氨酰-L-亮氨酰-苯丙氨酸（fMLP）作为O2-产生的刺激物时，观察到了这种抑制作用。然而，当用佛波酯（PMA）刺激时，脱敏细胞产生的O2-量与对照细胞一样多。脱敏细胞在短时间内（2-3分钟）预先暴露于小剂量的PMA。通过这个过程，细胞对WGA或fMLP诱导的O2-产生的活性几乎完全恢复。同样，细胞短期预先暴露于小剂量的PMA可消除3'-脱氮腺苷对WGA、fMLP或免疫复合物（IC）诱导的O2-产生的抑制作用。
