Király Gábor, Hargitai Zoltán, Kovács Ilona, Szemán-Nagy Gábor, Juhász István, Bánfalvi Gáspár
Department of Biotechnology and Microbiology, University of Debrecen, 1 Egyetem Square, Debrecen, 4010, Hungary.
Department of Surgery and Operative Techniques, University of Debrecen, 98 Nagyerdei körút, Debrecen, 4012, Hungary.
Pathol Oncol Res. 2019 Apr;25(2):625-633. doi: 10.1007/s12253-018-0492-7. Epub 2018 Nov 7.
Metastatic studies on rats showed that after subrenal implantation of tumor cells under the capsule of the kidney or subhepatic implantation under Glisson's capsule of the liver generated primary tumors in these organs. It was assumed that tumor cells that escaped through the disrupted peripheral blood vessels of primary tumors entered the peritoneal cavity, crossed the diaphragm, and appeared in the thoracal, primarily in the parathymic lymph nodes. This explanation did not answer the question whether distant lymph nodes were reached via the blood stream from the primary tumor or through the thoracal lymphatic vessels. In this work, we investigated the metastatic pathway in C3H/HeJ mice, after direct intraperitoneal administration of murine SCC VII cells bypassing the hematogenic spread of tumor cells. The direct pathway was also mimicked by intraperitoneal injection of Pelican Ink colloidal particles, which appeared in the parathymic lymph nodes, similarly to the tumor cells that caused metastasis in the parathymic lymph nodes and in the thymic tissue. The murine peritoneal-parathymic lymph node route indicates a general mechanism of tumor progression from the abdominal effusion. This pathway starts with the growth of abdominal tumors, continues as thoracal metastasis in parathymic lymph nodes and may proceed as mammary lymph node metastasis.
对大鼠的转移研究表明，在肾包膜下植入肿瘤细胞或在肝门脉周围包膜下肝下植入肿瘤细胞后，这些器官会产生原发性肿瘤。据推测，通过原发性肿瘤破裂的外周血管逃逸的肿瘤细胞进入腹腔，穿过膈肌，并出现在胸腔，主要是在胸腺旁淋巴结。这种解释没有回答远处淋巴结是通过原发性肿瘤的血流还是通过胸腔淋巴管到达的问题。在这项工作中，我们在直接腹腔内注射鼠SCC VII细胞绕过肿瘤细胞的血源性扩散后，研究了C3H/HeJ小鼠的转移途径。直接途径也通过腹腔内注射鹈鹕墨水胶体颗粒来模拟，这些颗粒出现在胸腺旁淋巴结中，类似于在胸腺旁淋巴结和胸腺组织中引起转移的肿瘤细胞。小鼠腹膜-胸腺旁淋巴结途径表明了肿瘤从腹腔积液进展的一般机制。该途径始于腹部肿瘤的生长，继续发展为胸腺旁淋巴结的胸腔转移，并可能发展为乳腺淋巴结转移。
