Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary.
Cancer Metastasis Rev. 2012 Jun;31(1-2):89-97. doi: 10.1007/s10555-011-9331-y.
Parathymic lymph nodes as potential sites of tumor progression have been neglected in humans. We have established a rat renal capsule-parathymic lymph node model to study in vivo metastasis. Epithelial liver carcinoma (HeDe) and mesenchymal mesoblastic nephroma (NeDe) cell lines have been established after inducing chemical carcinogenesis in newborn Fisher 344 inbred rats by N-nitrosodimethylamine. Implanting the exact number of tumor cells (HeDe, NeDe) under the renal capsule allowed the standardization and timing of metastatic development. Tumor cells released from the primary tumor in the peritoneal cavity were drained to the parathymic lymph nodes (PTNs) as sentinel lymph nodes. Similarly, tumor cells injected i.p. were engulfed by macrophages, drained through the transdiaphragmatic channels, and transported to the thoracal lymphatics, primarily to PTNs. Tumor cells after transdiaphragmic drainage can enter both anterior mammary and parathymic sentinel lymph nodes. The potential common origin can shed new light on the metastatic cell progression of PTNs and mammary tumors.
甲状旁腺淋巴结作为肿瘤进展的潜在部位在人类中被忽视了。我们建立了大鼠肾囊-甲状旁腺淋巴结模型，以研究体内转移。通过 N-亚硝基二甲胺诱导新生 Fisher 344 近交系大鼠化学致癌作用，建立了上皮性肝癌（HeDe）和间充质间胚细胞瘤（NeDe）细胞系。将确切数量的肿瘤细胞（HeDe、NeDe）植入肾囊下，可使转移性发展的标准化和定时化。从腹腔原发性肿瘤释放的肿瘤细胞被引流到甲状旁腺淋巴结（PTNs）作为前哨淋巴结。同样，腹腔内注射的肿瘤细胞被巨噬细胞吞噬，通过横膈膜通道引流，并运送到胸导管，主要是到 PTNs。横膈膜引流后的肿瘤细胞可以进入前乳和甲状旁腺前哨淋巴结。潜在的共同起源可以为甲状旁腺和乳腺肿瘤的转移细胞进展提供新的线索。
