Travis E L, Newman R A, Helbing S J
Int J Radiat Oncol Biol Phys. 1987 Sep;13(9):1355-9. doi: 10.1016/0360-3016(87)90229-x.
The ability of WR 2721 to protect endothelial cells and Type II cells in mouse lung after single doses of X rays was studied using specific assays of cell function to assess damage. The whole thorax of mice was exposed to a range of single doses of X rays either alone or 30 minutes after an i.p. injection of 400 mg/kg of WR 2721. Endothelial cell function was assayed by angiotensin converting enzyme (ACE) and Type II cell function by phosphatidylcholine and total protein present in lavage fluid 28 days after radiation. Similar protection factors (PFs) were found for the functional activity of both cell types, 1.2 and 1.24 for ACE and phosphatidylcholine respectively. These values were somewhat less than the PF of 1.37 for lethality from pneumonitis 7 to 9 months after irradiation for this mouse strain. The lack of a clear difference between the PFs for the functional activity of these two cell types suggests that neither the endothelial cell nor the Type II cell can be accepted or excluded as the target cell for radiation pneumonitis in lung.
使用细胞功能的特定检测方法来评估损伤，研究了WR 2721在单次X射线照射后对小鼠肺内皮细胞和II型细胞的保护能力。将小鼠的整个胸部单独暴露于一系列单次X射线剂量下，或在腹腔注射400 mg/kg的WR 2721后30分钟进行照射。通过血管紧张素转换酶（ACE）检测内皮细胞功能，通过辐射后28天灌洗液中的磷脂酰胆碱和总蛋白检测II型细胞功能。两种细胞类型的功能活性发现了相似的保护因子（PFs），ACE和磷脂酰胆碱的保护因子分别为1.2和1.24。这些值略低于该小鼠品系照射后7至9个月因肺炎致死的PF值1.37。这两种细胞类型功能活性的PFs之间缺乏明显差异，表明内皮细胞和II型细胞都不能被确定为肺放射性肺炎的靶细胞，也不能排除它们是靶细胞的可能性。
