Max Planck Institute for the Physics of Complex Systems, Dresden, Germany.
Center for Systems Biology Dresden, Dresden, Germany.
Eur J Neurosci. 2018 Dec;48(12):3597-3605. doi: 10.1111/ejn.14257.
The optic cup houses multipotent retinal progenitor cells that proliferate and differentiate to form the mature retina, containing five main types of neurons and a single glial cell type, the Müller cell. Progenitors of the zebrafish optic cup generate clones that vary regarding the number and types of neurons, a process we previously showed could be described by stochastic models. Here, we present data indicating that each retinal progenitor cell, in the 24 hrs post-fertilization optic cup, is predestined to form a single Müller cell. This striking fate assignment of Müller cells reveals a dual nature of retinal lineages where stochastic mechanisms produce variable numbers of neurons while there is a strong deterministic component governing the formation of glia cells. A possible mechanism for this stereotypic fate assignment could be the maintenance of a clonal backbone during retina development, which would be similar to invertebrate and rodent cortical neurogenesis.
视杯包含多能性视网膜祖细胞，这些细胞增殖和分化形成成熟的视网膜，包含五种主要类型的神经元和一种单一的神经胶质细胞类型，即 Muller 细胞。斑马鱼视杯的祖细胞产生的克隆在神经元的数量和类型上存在差异，我们之前的研究表明，这一过程可以用随机模型来描述。在这里，我们提供的数据表明，在受精后 24 小时的视杯内，每个视网膜祖细胞都注定要形成一个单一的 Muller 细胞。这种 Muller 细胞的惊人命运分配揭示了视网膜谱系的双重性质，其中随机机制产生可变数量的神经元，而有一个强烈的确定性成分控制着神经胶质细胞的形成。这种定型命运分配的一个可能机制可能是在视网膜发育过程中维持克隆骨干，这类似于无脊椎动物和啮齿动物的皮质神经发生。
