Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Department of Biomedical Engineering, University of Connecticut, Storrs, Mansfield, Connecticut, 06269.
J Biomed Mater Res A. 2019 Jan;107(1):81-91. doi: 10.1002/jbm.a.36534. Epub 2018 Nov 8.
A smart hydrogel with dual self-healing and autofluoresent functionalities is presented. The protein hydrogel is fabricated by denaturing bovine serum albumin in a basic environment. Upon gelation, autofluorescence is induced and the protein hydrogel can be excited by a wide range of spectrum, ranging from 320 to 520 nm. It was also found that the as-prepared autofluorescent protein hydrogel possessed rapid and repetitive self-healing capability. Without any external stimulus, more than 90% recovery of the mechanical strength can be obtained within 10 min after destruction. Moreover, the as-prepared hydrogel exhibits excellent biocompatibility and cell attachment property after its pH adjustment to neutral pH, while both autofluorescence and self-healing properties were still retained. This study suggests a promising means to prepare multi-functional protein hydrogel with dual physicochemical functionalities, which holds great potential in biomedical related applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 81-91, 2019.
一种具有双重自修复和自荧光功能的智能水凝胶被提出。该蛋白质水凝胶是通过在碱性环境中使牛血清白蛋白变性而制成的。凝胶化后，会产生自发荧光，并且蛋白质水凝胶可以被从 320nm 到 520nm 的宽光谱激发。还发现，所制备的自荧光蛋白质水凝胶具有快速和重复的自修复能力。在破坏后 10 分钟内，无需任何外部刺激，即可获得超过 90%的机械强度恢复。此外，在将水凝胶的 pH 值调整至中性 pH 值后，其表现出优异的生物相容性和细胞附着性能，同时仍保留自荧光和自修复性能。这项研究为制备具有双重物理化学功能的多功能蛋白质水凝胶提供了一种很有前途的方法，在生物医学相关应用中具有很大的潜力。 © 2018 威利父子出版公司。J 生物医学材料研究 A 部分：107A：81-91，2019.
