Shaanxi Key Laboratory of Degradable Biomedical Materials, Northwest University, Taibai North Road 229 Xi'an, Xi'an, Shaanxi, 710069, China.
Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, 710069, China.
Macromol Biosci. 2021 May;21(5):e2000396. doi: 10.1002/mabi.202000396. Epub 2021 Mar 10.
Protein-based hydrogels are important functional materials with many potential applications. However, the relatively small pore size and poor mechanical properties substantially limit their application. Here a superporous bovine serum albumin (BSA) hydrogel is prepared with high porosity and interconnectivity by using BSA and 1,2,7,8-diepoxyoctane (DEO). The equilibrium water contents of hydrogels can reach 76.5%. Moreover, the BSA hydrogels show excellent mechanical properties and excellent deformation recoverability, with a maximum compression modulus of 50 MPa at 75% strain and no residual strain generated after 500 cyclic compression tests. The resulting BSA hydrogel has excellent biocompatibility for cell adherence and is nonbiodegradable for 40 weeks. More importantly, the BSA hydrogel exhibits excellent hemostatic ability, with hemostatic times in a rabbit ear artery and rabbit liver of 33 and 28 s, respectively. Therefore, BSA hydrogels have potential applications as painless nonadherent wound dressings and implant materials for plastic surgery.
基于蛋白质的水凝胶是一种具有多种潜在应用的重要功能材料。然而，相对较小的孔径和较差的机械性能极大地限制了它们的应用。在这里，通过使用牛血清白蛋白（BSA）和 1,2,7,8-二环氧辛烷（DEO）制备了具有高孔隙率和互连性的超多孔 BSA 水凝胶。水凝胶的平衡含水量可达 76.5%。此外，BSA 水凝胶表现出优异的机械性能和出色的变形恢复能力，在 75%应变下最大压缩模量为 50 MPa，在 500 次循环压缩试验后没有产生残余应变。得到的 BSA 水凝胶具有良好的细胞黏附生物相容性，并且在 40 周内不可生物降解。更重要的是，BSA 水凝胶表现出优异的止血能力，在兔耳动脉和兔肝中的止血时间分别为 33 和 28 s。因此，BSA 水凝胶有望用作无痛非粘连伤口敷料和整形手术用植入材料。
