衰老对脓毒症大鼠肝脏碳水化合物代谢的影响。

Effect of aging on hepatic carbohydrate metabolism in septic rats.

作者信息

Schumer W, Kuttner R E

出版信息

J Gerontol. 1987 Sep;42(5):487-90. doi: 10.1093/geronj/42.5.487.

DOI:10.1093/geronj/42.5.487

Abstract

Aged individuals have diminished resistance to severe sepsis and septic shock. Past work with animals suggested that an important determinant of survival was the ability of the liver to supply glucose. In this study, young adult (3 to 4 months) and old (24 months) Fischer 344 rats were fasted and subjected to cecal incisions producing a rapidly lethal peritonitis. We then determined gluconeogenic intermediates in the liver. In the old rats with peritonitis, hexosemonophosphates (HMP) increased 50% relative to control liver, whereas in the young animals with peritonitis, the substrate decreased 50%. The accumulation of HMP in the old rat liver cells indicates a failure to dephosphorylate glucose-6-phosphate (G6P). This increase in HMP is associated with a decline in hepatic glucose-6-phosphatase (G6Pase), the final enzyme in the gluconeogenic pathway, and is reflected in a significant reduction in serum glucose in old Fischer 344 rats when compared to young Fischer rats.

摘要

老年个体对严重脓毒症和脓毒性休克的抵抗力下降。过去对动物的研究表明，生存的一个重要决定因素是肝脏提供葡萄糖的能力。在本研究中，将年轻成年（3至4个月）和老年（24个月）的Fischer 344大鼠禁食，并进行盲肠切开术，引发快速致死性腹膜炎。然后我们测定了肝脏中的糖异生中间产物。在患有腹膜炎的老年大鼠中，己糖单磷酸（HMP）相对于对照肝脏增加了50%，而在患有腹膜炎的年轻动物中，该底物减少了50%。老年大鼠肝细胞中HMP的积累表明6-磷酸葡萄糖（G6P）去磷酸化失败。HMP的这种增加与糖异生途径中的最终酶——肝葡萄糖-6-磷酸酶（G6Pase）的下降有关，并且与老年Fischer 344大鼠与年轻Fischer大鼠相比血清葡萄糖的显著降低有关。