Tuscher Jennifer J, Taxier Lisa R, Fortress Ashley M, Frick Karyn M
Department of Psychology, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, United States.
Department of Psychology, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, United States.
Neurobiol Learn Mem. 2018 Dec;156:103-116. doi: 10.1016/j.nlm.2018.11.002. Epub 2018 Nov 5.
The dorsal hippocampus (DH) and medial prefrontal cortex (mPFC) are brain regions essential for processing and storing episodic memory. In rodents, the DH has a well-established role in supporting the consolidation of episodic-like memory in tasks such as object recognition and object placement. However, the role of the mPFC in the consolidation of episodic-like memory tasks remains controversial. Therefore, the present study examined involvement of the DH and mPFC, alone and in combination, in object and spatial recognition memory consolidation in ovariectomized female mice. To this end, we utilized two types of inhibitory Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) to inactivate the DH alone, the mPFC alone, or both brain regions concurrently immediately after object training to assess the role of each region in the consolidation of object recognition and spatial memories. Our results using single and multiplexed DREADDS suggest that excitatory activity in the DH and mPFC, alone or in combination, is required for the successful consolidation of object recognition and spatial memories. Together, these studies provide critical insight into how the DH and mPFC work in concert to facilitate memory consolidation in female mice.
背侧海马体（DH）和内侧前额叶皮质（mPFC）是处理和存储情景记忆所必需的脑区。在啮齿动物中，DH在支持诸如物体识别和物体放置等任务中的类情景记忆巩固方面具有明确的作用。然而，mPFC在类情景记忆任务巩固中的作用仍存在争议。因此，本研究考察了DH和mPFC单独及联合参与去卵巢雌性小鼠物体和空间识别记忆巩固的情况。为此，我们使用了两种由设计药物特异性激活的抑制性设计受体（DREADDs），在物体训练后立即单独使DH失活、单独使mPFC失活或同时使两个脑区失活，以评估每个区域在物体识别和空间记忆巩固中的作用。我们使用单一和多重DREADDs的结果表明，DH和mPFC单独或联合的兴奋性活动是物体识别和空间记忆成功巩固所必需的。总之，这些研究为DH和mPFC如何协同作用促进雌性小鼠的记忆巩固提供了关键见解。
