Ariyasu R G, Ellisman M H
J Neurocytol. 1987 Apr;16(2):239-48. doi: 10.1007/BF01795307.
(Na+ + K+)ATPase-like immunoreactivity along the axolemma of sensory and motor neurons and the plasmalemma of Schwann cells from spinal roots of dystrophic mice (129 ReJ Dy/Dy) was determined using polyclonal antibodies specific for guinea pig renal (Na+ + K+)ATPase (GP-17), along with polyclonal (439-2) and monoclonal (9A5) antibodies specific for rat renal (Na+ + K+)ATPase. In normal and dystrophic mice, (Na+ + K+)ATPase-like immunoreactivity was observed along the axolemma at nodes of Ranvier using GP-17 and 439-2, each of which binds to isozymes of (Na+ + K+)ATPase composed of the alpha and alpha + forms of the catalytic subunit. Staining was not seen along the nodal axolemma with 9A5, a preparation that binds to the alpha form of the catalytic subunit. The terminal processes and microvilli of Schwann cells were stained using all three antibody probes. The axolemma of unensheathed axons in dystrophic mice was continuously and uniformly labelled with GP-17 and 439-2, but not 9A5. Concentrations of (Na+ + K+)ATPase-like immunoreactivity along Schwann cell processes were observed most often in areas adjacent to unensheathed axolemma. At heminodes, staining abruptly decreased along Schwann cell processes in areas that were separated from the unensheathed axolemma by other intervening Schwann cell processes. It was concluded from these data that in dystrophic mice (Na+ + K+)ATPase is uniformly distributed along unensheathed portions of axons without evidence of detectable focal concentrations of the enzyme, and that the catalytic subunit of (Na+ + K+)ATPase along unensheathed axons is distinct from the alpha form found in Schwann cells and other organs. In addition, (Na+ + K+)ATPase is concentrated along the plasmalemma of Schwann cells in regions of close apposition to axolemmal areas associated with large ionic fluxes.
利用针对豚鼠肾（Na⁺ + K⁺）ATP酶（GP - 17）的多克隆抗体以及针对大鼠肾（Na⁺ + K⁺）ATP酶的多克隆抗体（439 - 2）和单克隆抗体（9A5），测定了营养不良小鼠（129 ReJ Dy/Dy）脊髓神经根感觉神经元和运动神经元轴膜以及施万细胞质膜上的（Na⁺ + K⁺）ATP酶样免疫反应性。在正常和营养不良小鼠中，使用GP - 17和439 - 2在郎飞结处的轴膜上观察到（Na⁺ + K⁺）ATP酶样免疫反应性，这两种抗体均与由催化亚基的α和α + 形式组成的（Na⁺ + K⁺）ATP酶同工酶结合。用9A5（一种与催化亚基的α形式结合的制剂）未在结轴膜上观察到染色。施万细胞的终末突起和微绒毛用所有三种抗体探针均被染色。营养不良小鼠中无髓鞘轴突的轴膜被GP - 17和439 - 2连续且均匀地标记，但未被9A5标记。沿施万细胞突起的（Na⁺ + K⁺）ATP酶样免疫反应性浓度最常出现在与无髓鞘轴膜相邻的区域。在半结处，在被其他中间施万细胞突起与无髓鞘轴膜隔开的区域，沿施万细胞突起的染色突然减少。从这些数据得出的结论是，在营养不良小鼠中，（Na⁺ + K⁺）ATP酶沿轴突的无髓鞘部分均匀分布，没有该酶可检测到的局部浓度的证据，并且沿无髓鞘轴突的（Na⁺ + K⁺）ATP酶催化亚基与在施万细胞和其他器官中发现的α形式不同。此外，（Na⁺ + K⁺）ATP酶集中在施万细胞质膜上与伴有大量离子通量的轴膜区域紧密相邻的区域。
