Powell H C, Garrett R S, Kador P F, Mizisin A P
Department of Pathology (Neuropathology), University of California, San Diego, La Jolla 92093.
Acta Neuropathol. 1991;81(5):529-39. doi: 10.1007/BF00310134.
Aldose reductase was visualized by light and electron microscopy using a goat anti-rat antibody with immunoperoxidase and immunogold, respectively. Ouabain-sensitive, K(+)-dependent, p-nitro-phenylphosphatase, a component of (Na+, K+)-ATPase, was localized at the electron microscopic level by enzyme histochemistry using p-nitro-phenylphosphate as substrate. In peripheral nerve, spinal ganglia and roots, the Schwann cell of myelinated fibers was the principal site of aldose reductase localization. Immunostaining was intense in the paranodal region and the Schmidt-Lanterman clefts as well as in cytoplasm of the terminal expansions of paranodal myelin lamellae and the nodal microvilli. Schwann cell cytoplasm of unmyelinated fibers were faintly labelled. Endoneurial vessel endothelia, pericytes and perineurium failed to bind appreciable amounts of aldose reductase antibody. However, mast cell granules bound antibody strongly. In contrast, p-nitro-phenylphosphatase reaction product was detected in the nodal axolemma, terminal loops of Schwann cell cytoplasm and the innermost layer of perineurial cells. In endothelial cells, reaction product was localized on either the luminal or abluminal, or on both luminal and abluminal plasmalemma. Endothelial vesicular profiles were often loaded with reaction product. Occasional staining of myelin and axonal organelles was noted. Mast cells lacked reaction product.
分别使用免疫过氧化物酶和免疫金标记的山羊抗大鼠抗体，通过光学显微镜和电子显微镜观察醛糖还原酶。以对硝基苯磷酸为底物，通过酶组织化学在电子显微镜水平定位哇巴因敏感的、钾离子依赖性的对硝基苯磷酸酶，它是（钠，钾）-ATP酶的一个组成部分。在周围神经、脊髓神经节和神经根中，有髓纤维的施万细胞是醛糖还原酶定位的主要部位。在结旁区域、施密特-兰特尔曼切迹以及结旁髓鞘板末端膨大的细胞质和结部微绒毛中，免疫染色强烈。无髓纤维的施万细胞细胞质有微弱标记。神经内膜血管内皮细胞、周细胞和神经束膜未能结合可观量的醛糖还原酶抗体。然而，肥大细胞颗粒强烈结合抗体。相比之下，在结部轴膜、施万细胞细胞质的终末环和神经束膜细胞的最内层检测到对硝基苯磷酸酶反应产物。在内皮细胞中，反应产物定位于腔面膜或腔面膜和基底面膜上，或两者都有。内皮囊泡轮廓常常充满反应产物。偶尔观察到髓鞘和轴突细胞器有染色。肥大细胞缺乏反应产物。
