College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou 310000, China.
Zhejiang Institute for Food and Drug Control, Hangzhou 310000, China.
Food Chem. 2019 Mar 15;276:202-208. doi: 10.1016/j.foodchem.2018.09.152. Epub 2018 Sep 29.
A rapid and sensitive method was developed to determine 131 pesticides in tea by on-line gel permeation chromatography-gas chromatography tandem mass spectrometer (on-line GPC-GC-MS/MS) using graphitized multi-walled carbon nanotubes (MWCNTs) as dispersive solid phase extraction (DSPE) sorbent in sample pretreatment. Sample clean-up performance of various functionalized MWCNTs and their influences to recoveries were investigated. In the method validation, good linearity correlation coefficients (R2) were obtained, which were higher than 0.99 for all pesticides. The percent recoveries were 78.2%-113.9% at the spiked concentration levels of 20, 50 and 200 µg/kg with relative standard deviations (RSDs, n = 6) lower than 15.8%. Limits of detections (LODs) were in the range of 0.5-5.0 µg/kg. This method is simple and fast with relatively high sensitivity and can achieve the requirements of pesticides analysis.
建立了一种采用在线凝胶渗透色谱-气相色谱串联质谱法（在线 GPC-GC-MS/MS），以石墨化多壁碳纳米管（MWCNTs）为分散固相萃取（DSPE）吸附剂，对茶叶中 131 种农药进行检测的方法。研究了不同功能化 MWCNTs 的样品净化性能及其对回收率的影响。在方法验证中，所有农药的线性相关系数（R2）均高于 0.99。在 20、50 和 200μg/kg 的加标浓度水平下，回收率为 78.2%-113.9%，相对标准偏差（RSD，n=6）低于 15.8%。检出限（LODs）在 0.5-5.0μg/kg 范围内。该方法简单、快速，灵敏度相对较高，可满足农药分析的要求。
