Zhao Dong, Wang Lei, Shen Shuo, Lu Enyu, Feng Junlong, Bai Nima, Chen Hongyu, Li Wei
Academy of Agriculture and Forestry, Qinghai University, Xining, 810016, China.
Key Laboratory of Integrated Management of Agricultural Pest in Qinghai Province, Xining, 810016, China.
Heliyon. 2025 Jan 7;11(2):e41770. doi: 10.1016/j.heliyon.2025.e41770. eCollection 2025 Jan 30.
Trifluralin (FLL) is extensively used in rapeseed fields in the Qinghai-Tibet Plateau (QTP) region. However, the degradation kinetics of FLL in this area and its impact on environmental microbial communities are not yet known. To investigate the degradation patterns and ecological benefits of FLL, this study established a comprehensive method for detecting FLL residues and selected efficient degrading bacterial strains. Degradation experiments were conducted in two typical soil types of the QTP, and the dynamic changes in microbial communities were explored. The results indicated that FLL degradation in soils from two different regions of the QTP followed first-order kinetics, with half-lives of 25 and 39 days, respectively. The application of FLL at 173 g/ha significantly increased bacterial richness and diversity in the soils of both regions. Three efficient degrading strains were selected from soil samples: FL-3 () with a degradation rate of 80.81 %, FL-5 () at 51.18 %, and FL-6 () at 49.98 %. Moreover, the optimal degradation conditions for these strains were determined, and it was verified that they had no adverse effects on the germination and seedling growth of rapeseed, wheat, and barley. The findings of this study provide important data for the environmental risk assessment of FLL and suggest potential biological resources for the rational use and environmental remediation of this herbicide. These results are significant for developing safe use and environmental management strategies for FLL in the QTP.
氟乐灵（FLL）在青藏高原（QTP）地区的油菜田中广泛使用。然而，该地区氟乐灵的降解动力学及其对环境微生物群落的影响尚不清楚。为了研究氟乐灵的降解模式和生态效益，本研究建立了一种检测氟乐灵残留的综合方法，并筛选出高效降解菌株。在青藏高原的两种典型土壤类型中进行了降解实验，并探讨了微生物群落的动态变化。结果表明，青藏高原两个不同地区土壤中氟乐灵的降解遵循一级动力学，半衰期分别为25天和39天。以173 g/ha的剂量施用氟乐灵显著增加了两个地区土壤中细菌的丰富度和多样性。从土壤样品中筛选出三株高效降解菌株：降解率为80.81%的FL-3（）、降解率为51.18%的FL-5（）和降解率为49.98%的FL-6（）。此外，还确定了这些菌株的最佳降解条件，并验证了它们对油菜、小麦和大麦的发芽和幼苗生长没有不利影响。本研究结果为氟乐灵的环境风险评估提供了重要数据，并为该除草剂的合理使用和环境修复提供了潜在的生物资源。这些结果对于制定青藏高原氟乐灵的安全使用和环境管理策略具有重要意义。
