高效液相色谱-超高效液相色谱-串联质谱法同时测定食品中的叶绿酸铁钠和叶绿酸铜钠
Simultaneous determination of sodium iron chlorophyllin and sodium copper chlorophyllin in food using high-performance liquid chromatography and ultra-performance liquid chromatography-mass spectrometry.
机构信息
Department of Food Science, Sun Moon University, Asan, Chungchengnam-do 31460, Republic of Korea.
Department of Food Science, Sun Moon University, Asan, Chungchengnam-do 31460, Republic of Korea.
出版信息
Food Chem. 2019 Mar 15;276:390-396. doi: 10.1016/j.foodchem.2018.10.015. Epub 2018 Oct 4.
A simultaneous method for analyzing sodium iron chlorophyllin (SIC) and sodium copper chlorophyllin (SCC) using high-performance liquid chromatography was developed. This method employed an Inertsil ODS-2 column and diode array detection at 395 nm, using methanol-water (97:3 and 80:20, v/v) containing 1% acetic acid as the mobile phase. Liquid chromatography-tandem mass spectrometry was used to identify the main components of SIC and SCC as Fe-isochlorine e4 and Cu-isochlorine e4, respectively. The limits of detection and quantitation of SIC were 1.2 and 4.1 mg/kg, respectively, while those of SCC were 1.4 and 4.8 mg/kg, respectively. For intraday and interday tests, the SIC recoveries from candy ranged from 81% to 101%, while SCC recoveries ranged from 100% to 109%. The developed method can be applied to the rapid determination of SIC and SCC in candy.
建立了一种同时分析铁叶绿酸钠（SIC）和铜叶绿酸钠（SCC）的高效液相色谱法。该方法采用 Inertsil ODS-2 柱和二极管阵列检测器，检测波长为 395nm，流动相为甲醇-水（97:3 和 80:20，v/v），含 1%乙酸。采用液相色谱-串联质谱法鉴定 SIC 和 SCC 的主要成分分别为 Fe-类叶绿素 e4 和 Cu-类叶绿素 e4。SIC 的检测限和定量限分别为 1.2 和 4.1mg/kg，SCC 的检测限和定量限分别为 1.4 和 4.8mg/kg。在日内和日间测试中，SIC 从糖果中的回收率在 81%至 101%之间，而 SCC 的回收率在 100%至 109%之间。该方法可用于快速测定糖果中的 SIC 和 SCC。
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