Sim Sol, Kim Young-Min, Park Yeong-Ju, Siddiqui Mohammed Xain, Gang Yejin, Lee Jihyun, Lee Chan, Suh Hee-Jae
Department of Food Science, Sun Moon University, Asan, Chungchengnam-do 31460, Korea.
Department of Environmental Engineering, Daegu University, Gyeongsan 38543, Korea.
Foods. 2020 Oct 15;9(10):1473. doi: 10.3390/foods9101473.
Polyvinyl acetate (PVAc) is used in various adhesive, paint, and transparent tape applications. It is also used as a food additive in food manufacturing to make chewing gum and fruit and vegetable glazes; however, guidelines on the amount of food additives that is used have not yet been established. In this study, a method was developed for analysis of polyvinyl acetate (PVAc) using high-performance liquid chromatography with an evaporative light scattering detector (HPLC-ELSD) and pyrolyzer-gas chromatography-mass spectrometry (Py-GC-MS). The analytical methods were applied to commercially available chewing gum. In the HPLC-ELSD analysis, the linearity was acceptable (R > 0.999), and the limits of detection and quantification were 22.2 and 67.3 µg/mL, respectively. The accuracies of PVAc were 87-115% at spike levels of 200-1000 µg/mL for the intra- and inter-day tests. The contents of PVAc in the chewing gum samples were n.d. (not detected)-13.8 g/kg. The presence of PVAc in chewing gum was verified with Py-GC-MS analysis, finding the typical pyrolysates of PVAc, such as acetic acid, benzene, toluene, styrene, indane, naphthalene, and acenaphthene. The developed analytical methods can be applied for successful identification of PVAc in chewing gum.
聚醋酸乙烯酯（PVAc）用于各种胶粘剂、涂料和透明胶带应用中。它还在食品制造中用作食品添加剂，用于制作口香糖以及水果和蔬菜釉料；然而，关于其使用的食品添加剂用量指南尚未制定。在本研究中，开发了一种使用带蒸发光散射检测器的高效液相色谱法（HPLC - ELSD）和热解器 - 气相色谱 - 质谱联用仪（Py - GC - MS）分析聚醋酸乙烯酯（PVAc）的方法。这些分析方法应用于市售口香糖。在HPLC - ELSD分析中，线性良好（R > 0.999），检测限和定量限分别为22.2和67.3 µg/mL。在日内和日间测试中，加标水平为200 - 1000 µg/mL时，PVAc的回收率为87 - 115%。口香糖样品中PVAc的含量为未检出（n.d.）至13.8 g/kg。通过Py - GC - MS分析验证了口香糖中PVAc的存在，发现了PVAc的典型热解产物，如乙酸、苯、甲苯、苯乙烯、茚、萘和苊。所开发的分析方法可成功应用于口香糖中PVAc的鉴定。
