College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
Biosensors (Basel). 2022 Jan 19;12(2):52. doi: 10.3390/bios12020052.
Molecularly imprinted polymers (MIPs) specifically targeting pentachloronitrobenzene (PCNB) and containing silver nanoparticles have been prepared by free radical polymerization reaction using methyl methacrylate (MMA) as a functional monomer, PCNB as a template molecule, 1,4-butanedioldimethacrylate as a cross linker, lauroyl peroxide (LPO) as an initiator, and the silver nanoparticles with the best surface-enhanced Raman scattering (SERS) effect as SERS enhancement materials. Our results indicated that MIPs specifically recognize PCNB from complex matrices. The intensity of the PCNB characteristic peak was proportional to the concentration, with a linear range of 0.005 to 0.15 μg/mL and a limit of detection of 5.0 ng/mL. The recovery rates and relative standard deviation for the detection of PCNB spiked in the rice samples were from 94.4% to 103.3% and from 4.6% to 7.4%, respectively. The experimental results are consistent with those by the GC-MS method, indicating that the rapid detection of PCNB in food matrices by SERS-MIPs is reliable. In view of the insolubility of PCNB in water, oil-soluble silver nanoparticles were synthesized which can be expanded to detect oil-soluble toxic substances. For the first time, the proposed method provides a point-of-care and cost-effective tool for rapidly detecting PCNB in food matrices with high sensitivity and selectivity by employing SERS-MIPs method.
分子印迹聚合物（MIPs）特异性靶向五氯硝基苯（PCNB）并包含银纳米粒子，通过自由基聚合反应制备，甲基丙烯酸甲酯（MMA）用作功能单体，PCNB 用作模板分子，1,4-丁二醇二甲丙烯酸酯作为交联剂，过氧化月桂酰（LPO）作为引发剂，以及具有最佳表面增强拉曼散射（SERS）效果的银纳米粒子作为 SERS 增强材料。我们的结果表明，MIPs 可以特异性地从复杂基质中识别 PCNB。PCNB 特征峰的强度与浓度成正比，线性范围为 0.005 至 0.15 μg/mL，检测限为 5.0 ng/mL。在大米样品中检测 PCNB 加标时，回收率和相对标准偏差分别在 94.4%至 103.3%和 4.6%至 7.4%之间。实验结果与 GC-MS 方法一致，表明 SERS-MIPs 可用于可靠地快速检测食品基质中的 PCNB。鉴于 PCNB 在水中的不溶性，合成了油溶性银纳米粒子，可扩展到检测油溶性有毒物质。该方法首次为利用 SERS-MIPs 方法快速检测食品基质中的 PCNB 提供了一种具有高灵敏度和选择性的即时和经济有效的工具。
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