从影响体外淀粉消化率的水稻淀粉颗粒相关蛋白中提取和鉴定。
Extraction and characterization of starch granule-associated proteins from rice that affect in vitro starch digestibility.
机构信息
Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China; International Institute for Professional Education, Shanghai University of Finance and Economics, Shanghai 200083, China.
Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
出版信息
Food Chem. 2019 Mar 15;276:754-760. doi: 10.1016/j.foodchem.2018.10.042. Epub 2018 Oct 13.
Starch granule-associated proteins (SGAPs) including granule-surface proteins and granule-channel proteins in waxy, low- and high-amylose rice starch were extracted and identified. The in vitro digestibility of starch was investigated before and after the extraction of granule-channel proteins or total SGAPs. The results showed that 10 types of major differentially expressed proteins (DEPs) including 14-3-3-like protein and ribosomal protein were found among starches. In addition, the lack of only granule-channel proteins or total SGAPs led to significant and different changes in the levels of rapidly digestible starch, slowly digestible starch and resistant starch. Possible mechanisms are related to the accessibility of amylase into starch granules and structural properties of SGAPs. This study provides more information about DEPs in rice starch with different amylose content and supports further study on the relationship between SGAPs and in vitro starch digestibility.
蜡质、低直链淀粉和高直链淀粉稻米中淀粉粒相关蛋白（SGAPs），包括颗粒表面蛋白和颗粒通道蛋白，被提取并鉴定出来。在提取颗粒通道蛋白或总 SGAPs 前后，研究了淀粉的体外消化率。结果表明，在淀粉中发现了 10 种主要差异表达蛋白（DEPs），包括 14-3-3 样蛋白和核糖体蛋白。此外，仅缺失颗粒通道蛋白或总 SGAPs 会导致快速消化淀粉、缓慢消化淀粉和抗性淀粉的水平发生显著和不同的变化。可能的机制与淀粉酶进入淀粉颗粒的可及性和 SGAPs 的结构特性有关。本研究为不同直链淀粉含量的稻米淀粉中的 DEPs 提供了更多信息，并支持进一步研究 SGAPs 与体外淀粉消化率之间的关系。
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