Abe S, Yamaki A, Doi Y, Yoshioka I
Jpn J Med Sci Biol. 1987 Feb;40(1):1-13. doi: 10.7883/yoken1952.40.1.
Concentrated and purified Sabin and virulent strains of poliovirus types 1, 2 and 3 were inactivated with formalin at 37 C. By addition of 5.4 microM arildone, an antiviral agent, to the virus suspension, the stability of D antigen increased in both Sabin and virulent strains of all types, especially in virulent type 1 Mahoney strain. The drug had neither any inhibitory nor enhancing effect on the formalin inactivation. When antibody response was compared in guinea pigs, Sabin strains inactivated in the absence of arildone were less immunogenic against homotypic virulent strains than inactivated vaccine prepared from virulent strains. On the other hand, Sabin strains inactivated in the presence of arildone were equally immunogenic. These results indicate that it is possible to prepare from Sabin strains a potent and safe inactivated vaccine having an immunogenicity comparable to that prepared from virulent strains.
1型、2型和3型脊髓灰质炎病毒的浓缩纯化萨宾株和强毒株在37℃下用福尔马林灭活。通过向病毒悬液中添加5.4微摩尔的抗病毒药物阿里多，所有类型的萨宾株和强毒株中D抗原的稳定性均有所增加，尤其是强毒株1型马奥尼株。该药物对福尔马林灭活既无抑制作用也无增强作用。当在豚鼠中比较抗体反应时，在没有阿里多的情况下灭活的萨宾株对同型强毒株的免疫原性低于由强毒株制备的灭活疫苗。另一方面，在有阿里多的情况下灭活的萨宾株具有同等的免疫原性。这些结果表明，有可能从萨宾株制备出一种高效且安全的灭活疫苗，其免疫原性与由强毒株制备的疫苗相当。
