Zhou L F, Qi S Q, Lei H P
J Ethnopharmacol. 1987 Jun;20(1):39-43. doi: 10.1016/0378-8741(87)90117-6.
Rats were treated orally with gossypol acetic acid at 30 or 10 mg/kg daily, 6 days a week, for 8, 12, 14 or 16 weeks. At the end of each treatment regimen, treated rats and an equal number of control rats were killed for histological and histochemical studies. From 8 weeks onward, as a result of the treatment, the tubular lumen of the corpus epididymides became narrowed with thickened pseudostratified epithelium and there was a reduction in the amount of spermatozoa. There was an increase in esterase, alkaline phosphatase, acid phosphatase and ATPase activity. These changes increased in intensity with the duration of treatment. Scanning electron microscopic examinations of the corpus epididymides of rats treated for 16 weeks, compared with those of controls, revealed similar changes, namely, narrowing of the tubular lumen, thickening of the pseudostratified epithelium and reduction in the number of spermatozoa.
大鼠每天经口给予醋酸棉酚，剂量为30毫克/千克或10毫克/千克，每周6天，持续8、12、14或16周。在每个治疗方案结束时，处死接受治疗的大鼠和数量相等的对照大鼠，进行组织学和组织化学研究。从8周起，由于治疗，附睾体的管腔变窄，假复层上皮增厚，精子数量减少。酯酶、碱性磷酸酶、酸性磷酸酶和ATP酶活性增加。这些变化随着治疗时间的延长而加剧。与对照组相比，对接受16周治疗的大鼠附睾体进行扫描电子显微镜检查，发现了类似的变化，即管腔变窄、假复层上皮增厚和精子数量减少。
