Taylor Joseph M, Hughes Andrew T, Milan Anna M, Rudge James, Davison Andrew S, Ranganath Lakshminarayan R
Department of Clinical Biochemistry & Metabolic Medicine, Liverpool Clinical Laboratories, Royal Liverpool University Hospital, Liverpool, L7 8XP, UK.
Musculoskeletal Biology 1, Institute of Aging and Chronic Disease, University of Liverpool, Liverpool, L7 8TX, UK.
Bioanalysis. 2018 Dec;10(23):1919-1932. doi: 10.4155/bio-2018-0193. Epub 2018 Nov 6.
Alkaptonuria is a disorder of tyrosine metabolism where elevated circulating homogentisic acid causes progressive dysfunction of collagenous tissues. Logistical, financial and patient experience concerns with collection and transport of specimens to central laboratories makes evaluation of microsampling attractive.
Volumetric absorptive microsampling devices were evaluated for accuracy, precision and drying time. Elution methods were evaluated for several urinary metabolites of interest and stability assessed under multiple conditions. Comparison was performed between dried and liquid specimens via LC-MS/MS.
Volumetric absorptive microsampling was found to be highly accurate and precise. Elution methods showed good recovery and reproducibility. Dried and liquid samples compared favorably. Analyte stability was variable, presenting barriers to implementation into routine use in this context.
黑尿症是一种酪氨酸代谢紊乱疾病，循环中尿黑酸水平升高会导致胶原组织进行性功能障碍。将样本采集和运输至中心实验室存在后勤、财务及患者体验方面的问题，这使得微量采样评估颇具吸引力。
对体积吸收式微量采样装置的准确性、精密度和干燥时间进行评估。对几种感兴趣的尿液代谢物的洗脱方法进行评估，并在多种条件下评估稳定性。通过液相色谱-串联质谱法对干燥样本和液体样本进行比较。
发现体积吸收式微量采样具有高度准确性和精密度。洗脱方法显示出良好的回收率和重现性。干燥样本和液体样本表现良好。分析物稳定性存在差异，这在这种情况下构成了常规应用的障碍。
