Servicio de Bioquímica Clínica, Unidad de Cuantificación y Caracterización Molecular, Hospital Universitario Ramón y Cajal, Spain; Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Spain; CIBER de Fisiología de la Obesidad y Nutrición (CIBERobn), ISCIII, Spain.
Servicio de Bioquímica Clínica, Unidad de Cuantificación y Caracterización Molecular, Hospital Universitario Ramón y Cajal, Spain.
J Nutr Biochem. 2019 Jan;63:197-205. doi: 10.1016/j.jnutbio.2018.09.026. Epub 2018 Oct 11.
The evaluation of the benefits of omega-3 fatty acid supplementation in humans requires the identification and characterization of suitable biomarkers of its incorporation in the body. The reference method for the evaluation of omega-3, gas chromatography, is difficult to apply in clinical practice because of its low throughput and does not provide information about the incorporation of specific fatty acids in lipid species and the potential effects of supplementation on lipid classes. We used a quantitative lipidomic approach to follow the incorporation of omega-3 fatty acids into plasma lipids in cystic fibrosis patients (n=50) from a randomized controlled clinical trial after the supplementation of seaweed oil enriched with docosahexaenoic acid (DHA). Lipidomic analysis accurately showed the distribution of fatty acids in different lipid classes after omega-3 supplementation, and the performance in determining the compliance to supplementation was similar to that of gas chromatography coupled to mass spectrometry. Twelve months after fatty acid supplementation, DHA was predominantly incorporated into highly unsaturated cholesteryl esters (110.9±16.2 vs. 278.6±32.6 μM, mean±S.E.M.) and phosphatidylcholine (142.4±11.9 vs. 272.9±21.4 μM) and, to a lesser extent, into phosphatidylethanolamine (9.4±0.8 vs. 15.5±1.5 μM) and triglycerides (0.4±0.04 vs. 1.1±0.12 μM). In addition, a technique was developed for the fast measurement of the DHA/arachidonic acid ratio to simplify the follow-up of nutritional intervention with DHA-enriched foods. We conclude that lipidomics is a suitable approach for monitoring the incorporation of omega-3 fatty acids in nutritional studies.
评估ω-3 脂肪酸补充剂在人体中的益处需要确定和表征其在体内的合适生物标志物。评估ω-3 的参考方法——气相色谱法，由于其低通量，并且不能提供关于特定脂肪酸在脂质种类中的掺入以及补充对脂质类别的潜在影响的信息，因此在临床实践中难以应用。我们使用定量脂质组学方法来跟踪囊性纤维化患者（n=50）在补充富含二十二碳六烯酸（DHA）的海藻油后的血浆脂质中ω-3 脂肪酸的掺入。脂质组学分析准确地显示了ω-3 补充后脂肪酸在不同脂质类别的分布，其在确定补充依从性方面的性能与气相色谱法-质谱法相似。在脂肪酸补充 12 个月后，DHA 主要掺入高度不饱和的胆甾醇酯（110.9±16.2 与 278.6±32.6 μM，平均值±S.E.M.）和磷脂酰胆碱（142.4±11.9 与 272.9±21.4 μM），并且在较小程度上掺入磷脂酰乙醇胺（9.4±0.8 与 15.5±1.5 μM）和甘油三酯（0.4±0.04 与 1.1±0.12 μM）。此外，还开发了一种快速测量 DHA/花生四烯酸比值的技术，以简化富含 DHA 的食物的营养干预的随访。我们得出结论，脂质组学是监测营养研究中ω-3 脂肪酸掺入的合适方法。
