Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico.
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico.
Life Sci. 2019 Jan 1;216:22-28. doi: 10.1016/j.lfs.2018.11.017. Epub 2018 Nov 8.
Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats.
We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR.
Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared.
These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.
妊娠是一个心血管发生深刻变化的生理阶段，导致低血压。先兆子痫（PE）逆转这些正常变化，导致高血压。肾素-血管紧张素系统（RAS）与 PE 的发生有关。已经报道了该系统中的一种新型受体，即前肾素/肾素受体（PRR），在肾脏和心血管疾病中有多种作用。然而，在与高血压密切相关的组织中，PRR 是否在正常或 PE 合并妊娠期间改变其表达或被激活尚不清楚。因此，本工作的目的是描述 PRR 在正常和高血压妊娠大鼠中的表达。
我们使用了肾下腹主动脉缩窄（SRAC）大鼠模型。心房、室间隔和心室心脏组织、主动脉和肾组织样本被匀浆，并使用抗 PRR 和抗 PLZF 抗体进行免疫印迹。我们还通过 RT-PCR 测量基因表达。
在 SRAC 妊娠大鼠中观察到高血压和蛋白尿。在非妊娠、非 SRAC 妊娠大鼠中，PRR 在心脏、主动脉和肾脏组织中的基因和蛋白表达均高于非妊娠大鼠。PE 可诱导心脏组织中 PRR 的高表达，而在主动脉和肾脏中则降低 PRR 的表达。PLZF，PRR 功能的标志物，仅在非 SRAC 妊娠大鼠的主动脉和肾脏中增加。在 SRAC 妊娠大鼠中，PLZF 的增加消失了。
这些发现表明，PRR 在与心血管功能相关的组织中，在妊娠和 PE 期间的表达不同，并提示受体可能在正常和先兆子痫妊娠大鼠中参与其中。
