Laboratory of Molecular Radiobiology and Gerontology, Institute of Biology of Komi Science Center of Ural Division of Russian Academy of Sciences, Syktyvkar, Russia; Department of Ecology, Syktyvkar State University, Syktyvkar, Russia.
Laboratory of Molecular Radiobiology and Gerontology, Institute of Biology of Komi Science Center of Ural Division of Russian Academy of Sciences, Syktyvkar, Russia.
Exp Gerontol. 2019 Mar;117:61-71. doi: 10.1016/j.exger.2018.11.005. Epub 2018 Nov 9.
Gene expression is much altered in aging. We observed age-dependent decline of core clock genes' expression in the whole body of the fruit fly. We hypothesized that inducible overexpression of clock genes (cry, per, tim, cyc and Clk) in the nervous system can improve healthspan of D. melanogaster. We studied the lifespan of transgenic Drosophila and showed life extension for cry, per, cyc and tim genes. It was also the significant positive changes in the stress-resistance of flies overexpressing core clock genes in conditions of hyperthermia, hyperoxia, starvation and persistent lighting. The overexpression of per and cry restore circadian rhythms of locomotor activity. The results presented support the hypotheses that the compensation of circadian oscillator genes expression can improve the healthspan in Drosophila melanogaster.
基因表达在衰老过程中发生了很大的改变。我们观察到果蝇全身核心时钟基因表达随年龄的依赖性下降。我们假设在神经系统中诱导过表达时钟基因（cry、per、tim、cyc 和 Clk）可以延长 D. melanogaster 的健康寿命。我们研究了转基因果蝇的寿命，并显示 cry、per、cyc 和 tim 基因的寿命延长。在高温、高氧、饥饿和持续光照条件下，过表达核心时钟基因的果蝇对压力的抵抗力也有显著的积极变化。per 和 cry 的过表达恢复了运动活性的昼夜节律。这些结果支持了这样的假设，即补偿昼夜振荡器基因表达可以改善 D. melanogaster 的健康寿命。
