Central European Institute of Technology (CEITEC), Masaryk University, 625 00 Brno, Czech Republic.
Institute of Histology and Embryology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic.
J Colloid Interface Sci. 2019 Mar 1;537:20-27. doi: 10.1016/j.jcis.2018.11.005. Epub 2018 Nov 3.
Palladium and silver nanoparticles (NPs) anchored at the outer surface of ferritin form stable suspension of non-coated particles that possess several catalytic and enzymomimetic activities. These activities are strongly affected by detergents that significantly influence the reaction efficiency and specificity. Reductive dehalogenation of various azo dye substrates shows strong differences in reactivity for each substrate-detergent pair. Reductive dehalogenation is negatively influenced by cationic detergents while catalytic depropargylation is severely impaired by polyethylene oxide containing detergents that is an important finding in respect to potential biorthogonal applications. Moreover, Suzuki-Miyaura reaction is promoted by polyethylene oxide containing detergents but some of them also facilitate dehalogenation. Enzymomimetic peroxidase activity of silver NPs can be detected only in presence of sodium dodecyl sulfate (SDS) while peroxidase activity of palladium NPs is enhanced by SDS and sodium deoxycholate.
钯和银纳米粒子（NPs）锚定在铁蛋白的外表面上形成非涂层颗粒的稳定悬浮液，这些颗粒具有多种催化和酶模拟活性。这些活性受洗涤剂的强烈影响，这显著影响反应效率和特异性。各种偶氮染料底物的还原脱卤反应对每个底物-洗涤剂对表现出强烈的反应性差异。还原脱卤反应受到阳离子洗涤剂的负面影响，而含有聚氧乙烯的洗涤剂严重损害催化炔丙基化反应，这是关于潜在的生物正交应用的一个重要发现。此外，含有聚氧乙烯的洗涤剂促进Suzuki-Miyaura 反应，但其中一些也促进脱卤反应。只有在存在十二烷基硫酸钠（SDS）的情况下，才能检测到银 NPs 的酶模拟过氧化物酶活性，而 SDS 和脱氧胆酸钠增强了钯 NPs 的过氧化物酶活性。
