Tagele Setu Bazie, Lee Hyun Gu, Kim Sang Woo, Lee Youn Su
Department of Applied Plant Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
J Microbiol Biotechnol. 2019 Jan 28;29(1):66-78. doi: 10.4014/jmb.1808.08026.
In this study, strain KNU17Pc1 was tested for its antifungal activity against AG-1(IA), which causes banded leaf and sheath blight (BLSB) of maize. KNU17Pc1 was tested further for its broad-spectrum antifungal activity and in vitro plant growth promoting (PGP) traits. In addition, the in vivo effects of KNU17Pc1 on reduction of BLSB severity and seedling growth promotion of two maize cultivars under greenhouse conditions were investigated. On the basis of multilocus sequence analysis (MLSA), KNU17Pc1 was confirmed as subsp. . The study revealed that KNU17Pc1 had strong in vitro antifungal activity and was effective toward all in vitro PGP traits except phosphate solubilization. In this study, for the first time, a strain of against , , f.sp. , and has been reported. Further biochemical studies showed that KNU17Pc1 was able to produce both types of phenazine derivatives, PCA and 2-OH-PCA. In addition, solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) analysis identified 13 volatile organic compounds (VOCs) in the TSB culture of KNU17Pc1, 1-undecene being the most abundant volatile. Moreover, for the first time, Octamethylcyclotetrasiloxan (D4), dimethyl disulfide, 2-methyl-1,3-butadiene and 1-undecene were detected in . Furthermore, this study reported for the first time the effectiveness of to control BLSB of maize. Hence, further studies are necessary to test the effectiveness of KNU17Pc1 under different environmental conditions so that it can be exploited further for biocontrol and plant growth promotion.
在本研究中，对菌株KNU17Pc1针对引起玉米条斑叶枯病（BLSB）的AG-1(IA)的抗真菌活性进行了测试。进一步测试了KNU17Pc1的广谱抗真菌活性及体外促植物生长（PGP）特性。此外，还研究了KNU17Pc1在温室条件下对两个玉米品种减轻条斑叶枯病严重程度和促进幼苗生长的体内效应。基于多位点序列分析（MLSA），KNU17Pc1被确认为亚种。研究表明，KNU17Pc1具有很强的体外抗真菌活性，对除解磷外的所有体外PGP特性均有效。在本研究中，首次报道了一株 对 、 、 致病型 、 及 的菌株。进一步的生化研究表明，KNU17Pc1能够产生两种类型的吩嗪衍生物，即PCA和2-OH-PCA。此外，固相微萃取-气相色谱-质谱（SPME-GC-MS）分析在KNU17Pc1的胰蛋白胨大豆肉汤（TSB）培养物中鉴定出13种挥发性有机化合物（VOC），其中1-十一碳烯是最丰富的挥发性物质。此外，首次在 中检测到八甲基环四硅氧烷（D4）、二甲基二硫、2-甲基-1,3-丁二烯和1-十一碳烯。此外，本研究首次报道了 对防治玉米条斑叶枯病的有效性。因此，有必要进一步研究KNU17Pc1在不同环境条件下的有效性，以便进一步将其用于生物防治和促进植物生长。
