Hu An, Liu Hai-Bin, Mlynski Robert, Plontke Stefan, Zhang Jing-Fei, Dai Wei-Jun, Duan Jun-Li, Fan Jing-Ping, Zheng Hong-Liang, Xu Wei-Hua, Chen Xiao-Ping, Huang Jing-Juan
Department of Otolaryngology, Gongli Hospital, Second Military Medical University, Pudong New Area Miaopu Road 219, Shanghai, China.
Department of Otorhinolaryngology, Head and Neck Surgery "Otto Koerner", Rostock University Medical Center Gertrudenstraße 9, 18057 Rostock, Germany.
Am J Transl Res. 2018 Oct 15;10(10):3099-3110. eCollection 2018.
Postoperative pain has well defined and is perceived by patients as one of the most obnoxious aspects of surgical pain. The aim of this study was to determine whether the combination of Therapeutic ultrasound (TUS) and Curcumin (CUR) resulted in an enhancement of their pain relieving activities in a rat model of postoperative pain. We explored the effect of these treatment and their interaction with signal transduction pathways involved in inflammatory. In this study, TUS and CUR alone or in combination were administered prior to or simultaneously with or after the incisional surgery. At the start time of administration, we observed that the TUS plus CUR treatment reduced the mean paw withdrawal threshold more efficiently than CUR alone. Then we demonstrated that TUS potentiates the antinociceptive effect of CUR in a rat model of chronic postoperative pain and that the combination could facilitate the recovery of surgical pain. However, preventive value was not statistically significant when the treatments were given prior to the incisional surgery. We provide evidence that TUS plus CUR administrations were safe and significantly reduced the ED50 compared to treatment with the single CUR treatment in rats. TUS plus CUR administrations decreases incisional surgery induced activation of inflammatory cells and down-regulation of chemokines and proinflammatory cytokines, MCP-1, MIP-1α, IL-1β, and TNF-α through regulating Sirt1/NF-κB signaling pathway. Taken together, our results indicate that the combinations of TUS and CUR can be more effective in the anti-nociceptive effects than the treatment with CUR alone.
术后疼痛有明确的定义，且患者将其视为手术疼痛中最令人厌恶的方面之一。本研究的目的是确定治疗性超声（TUS）与姜黄素（CUR）联合使用是否能增强它们在大鼠术后疼痛模型中的止痛活性。我们探究了这些治疗方法的效果以及它们与炎症相关信号转导通路的相互作用。在本研究中，单独或联合使用TUS和CUR，在切口手术前、同时或之后给药。在给药开始时，我们观察到TUS加CUR治疗比单独使用CUR更有效地降低了平均爪部退缩阈值。然后我们证明，在大鼠慢性术后疼痛模型中，TUS增强了CUR的抗伤害感受作用，并且联合使用可以促进手术疼痛的恢复。然而，在切口手术前给药时，预防价值在统计学上并不显著。我们提供的证据表明，与大鼠单独使用CUR治疗相比，TUS加CUR给药是安全的，并且显著降低了半数有效剂量（ED50）。TUS加CUR给药通过调节Sirt1/NF-κB信号通路，减少了切口手术诱导的炎症细胞活化以及趋化因子和促炎细胞因子MCP-1、MIP-1α、IL-1β和TNF-α的下调。综上所述，我们的结果表明，TUS和CUR联合使用在抗伤害感受作用方面比单独使用CUR治疗更有效。
