识别和综合分析 ER 阳性亚型乳腺癌中关键差异表达的环状 RNA。
Identification and integrated analysis of key differentially expressed circular RNAs in ER-positive subtype breast cancer.
机构信息
Department of Breast Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, PR China.
出版信息
Epigenomics. 2019 Feb;11(3):297-321. doi: 10.2217/epi-2018-0147. Epub 2018 Nov 12.
AIM
To systematically profile and characterize the circular RNA (circRNA) expression pattern in estrogen receptor (ER)-positive breast cancer (BC).
MATERIALS & METHODS: CircRNA expression profile was performed in ER-positive BC and adjacent nontumor tissues. The differentially expressed circRNAs (DECs) was analyzed by bioinformatics. The analysis findings were validated by quantitative real-time PCR.
RESULTS
In total, 3653 DECs were detected in our ER-positive BC compared with the control. Bioinformatics analysis showed that some pathways related to cancer, especially BC, were significantly enriched. Additionally, hsa_circ_0087378 was validated to be downregulated in ER-positive BC and the hsa_circ_0087378-miR-1260b-SFRP1 axis was proposed to be a key regulatory pathway.
CONCLUSION
This study revealed the general expression characteristics of specific DECs in ER-positive BC and hsa_circ_0087378 might be a promising candidate target.
目的
系统分析雌激素受体（ER）阳性乳腺癌（BC）中环状 RNA（circRNA）的表达模式和特征。
材料与方法
在 ER 阳性 BC 及相邻非肿瘤组织中进行 circRNA 表达谱分析。通过生物信息学分析差异表达的 circRNAs（DECs）。采用实时定量 PCR 对分析结果进行验证。
结果
与对照组相比，我们在 ER 阳性 BC 中检测到 3653 个 DECs。生物信息学分析表明，一些与癌症相关的通路，尤其是 BC，显著富集。此外，hsa_circ_0087378 在 ER 阳性 BC 中表达下调，提出 hsa_circ_0087378-miR-1260b-SFRP1 轴可能是一个关键的调控通路。
结论
本研究揭示了 ER 阳性 BC 中特定 DECs 的一般表达特征，hsa_circ_0087378 可能是一个很有前途的候选靶标。
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