Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan.
Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan; Research Center for Animal Biologics, National Pingtung University of Science and Technology, Pingtung, 912, Taiwan.
Fish Shellfish Immunol. 2019 Jan;84:1050-1058. doi: 10.1016/j.fsi.2018.11.017. Epub 2018 Nov 9.
The probiotic efficiencies of the mixed probiotics containing Lactobacillus pentosus BD6, Lac. fermentum LW2, Bacillus subtilis E20, and Saccharomyces cerevisiae P13 for shrimp growth and health status improvement were better than those when using single probiotics. The probiotic mixture at a level of 10 colony-forming units (cfu) (kg diet) and the diets containing BD6 and E20 at 10 cfu (kg diet) significantly improved the growth and health status of shrimp, whereas the diets containing P13 or LW2 did not significantly affect the growth of shrimp. No significant difference in the carcass composition was recorded among the control and treatments. After 56 days of feeding, shrimp fed the diet containing the probiotic mixture (10∼10 cfu (kg diet)) had higher survival after injection with the V. alginolyticus, but 10 cfu (kg diet) of single probiotics (except for S. cerevisiae P13) had to be administered to improve shrimp survival. The better disease resistance of shrimp in groups fed the probiotic mixture might have been due to increased phenoloxidase activity, respiratory bursts, and lysozyme activity of hemocytes. Therefore, we considered that the probiotic mixture could adequately provide probiotic efficiency for white shrimp, and a diet containing 10 cfu (kg diet) probiotic mixture is recommended.
混合益生菌（包含戊糖片球菌 BD6、发酵乳杆菌 LW2、枯草芽孢杆菌 E20 和酿酒酵母 P13）对虾生长和健康状况的益生菌效率优于单一益生菌。益生菌混合物水平为 10⁻¹⁰ 菌落形成单位（cfu）（kg 饲料）和饲料中 BD6 和 E20 的含量为 10⁻¹⁰ cfu（kg 饲料）可显著改善虾的生长和健康状况，而饲料中含有 P13 或 LW2 对虾的生长没有显著影响。对照组和处理组之间的虾体成分没有显著差异。在喂养 56 天后，喂食含有益生菌混合物（10∼10⁻¹⁰ cfu（kg 饲料））的饲料的虾在注射 Alg 溶藻弧菌后的存活率更高，但要提高虾的存活率，必须添加 10⁻¹⁰ cfu（kg 饲料）的单一益生菌（酿酒酵母 P13 除外）。摄食益生菌混合物的虾的抗病能力更好，可能是由于酚氧化酶活性、呼吸爆发和血细胞溶菌酶活性增加。因此，我们认为益生菌混合物可以为南美白对虾提供足够的益生菌效率，建议使用含有 10⁻¹⁰ cfu（kg 饲料）益生菌混合物的饲料。
