School of Biomedical Engineering, Capital Medical University, Beijing 100069, China.
Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Capital Medical University, Beijing 100069, China.
Biomed Res Int. 2018 Oct 21;2018:4862309. doi: 10.1155/2018/4862309. eCollection 2018.
The flow field of aqueous humor correlates to the stiffness of iris pigment epithelium (IPE) which acts as a wall of posterior chamber. We focus on the variations of IPE stiffness in a rat ocular hypertension (OHT) model, so as to prepare for exploring the mechanism of duration of OHT.
Episcleral venous cauterization (EVC) was applied on one eye of male adult Sprague-Dawley rats to induce chronic high intraocular pressure. According to the duration of OHT (0, 1, 2, 4, and 8 weeks), rats were randomly divided into Gw0, Gw1, Gw2, Gw4, and Gw8. Atomic force microscope (AFM) analysis was applied to test IPE stiffness in three regions: iris root, mid-periphery, and pupillary-margin in each group. Histological changes of IPE were also examined in Gw4 and Gw8.
There was an overall growing tendency of IPE stiffness in EVC eye. IPE in EVC eye was significantly stiffer than fellow eye in Gw2, Gw4, and Gw8 (in iris root, mid-periphery, and pupillary-margin, p<0.05). IPE in EVC eye in pupillary-margin was significantly stiffer than iris root in Gw4 and Gw8 (p<0.05). In EVC eye, IPE becomes thinner and IPE cell density decreases.
IPE stiffness increases gradually with the duration of chronic high intraocular pressure.
房水的流动场与作为后房壁的虹膜色素上皮 (IPE) 的硬度相关。我们专注于在大鼠眼高压 (OHT) 模型中 IPE 硬度的变化，以便为探索 OHT 持续时间的机制做准备。
对雄性成年 Sprague-Dawley 大鼠的一只眼施行巩膜静脉烧灼术 (EVC) 以诱导慢性高眼压。根据 OHT 的持续时间 (0、1、2、4 和 8 周)，大鼠被随机分为 Gw0、Gw1、Gw2、Gw4 和 Gw8 组。原子力显微镜 (AFM) 分析用于测试每组中虹膜根部、中周边和瞳孔缘三个区域的 IPE 硬度。还在 Gw4 和 Gw8 检查了 IPE 的组织学变化。
EVC 眼中 IPE 硬度呈总体增长趋势。EVC 眼中的 IPE 在 Gw2、Gw4 和 Gw8 时明显比对侧眼硬 (在虹膜根部、中周边和瞳孔缘，p<0.05)。Gw4 和 Gw8 时 EVC 眼中的 IPE 在瞳孔缘比虹膜根部硬 (p<0.05)。在 EVC 眼中，IPE 变薄，IPE 细胞密度降低。
随着慢性高眼压持续时间的延长，IPE 硬度逐渐增加。
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