来自尿路感染的尿路致病性大肠杆菌分离株的分子分析
Molecular Analysis of Uropathogenic E.coli Isolates from Urinary Tract Infections.
作者信息
Valadbeigi Hassan, Esmaeeli Elham, Ghafourian Sobhan, Maleki Abbas, Sadeghifard Nourkhoda
机构信息
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
出版信息
Infect Disord Drug Targets. 2019;19(3):322-326. doi: 10.2174/1871526518666181113091322.
INTRODUCTION
The aim of the current study was to investigate the prevalence of virulence genes in uropathogenic Escherichia coli (UPEC) isolates in Ilam.
MATERIALS AND METHODS
For this purpose, a total of 80 UPEC isolates were collected for patients with UTIs during a 6 months period. The multiplex polymerase chain reaction (multiplex PCR) was used to detect the papEF, fimH, iucD, hlyA, fyuA, and ompT genes.
RESULTS
The prevalence of fimH, papEF, iucD, fyuA, hlyA, hlyA, and ompT genes were 87.5%, 47.5%, 60%, 67.5%, 27.5%, 47.5% and 71.2%, respectively. Among all of the isolates, 27 profiles were obtained.
CONCLUSION
Our findings demonstrated that the most prevalence was found for fimH, and different distribution of virulence genes suggested different ability of pathogenicity.
引言
本研究的目的是调查伊拉姆地区尿路致病性大肠杆菌（UPEC）分离株中毒力基因的流行情况。
材料与方法
为此，在6个月期间共收集了80株UTIs患者的UPEC分离株。采用多重聚合酶链反应（多重PCR）检测papEF、fimH、iucD、hlyA、fyuA和ompT基因。
结果
fimH、papEF、iucD、fyuA、hlyA、hlyA和ompT基因的流行率分别为87.5%、47.5%、60%、67.5%、27.5%、47.5%和71.2%。在所有分离株中，获得了27种谱型。
结论
我们的研究结果表明，fimH的流行率最高，毒力基因的不同分布表明致病性的不同能力。
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