Rus H G, Niculescu F, Vlaicu R
Medical Clinic No.1, Cluj-Napoca, Romania.
Clin Immunol Immunopathol. 1988 Sep;48(3):307-16. doi: 10.1016/0090-1229(88)90024-4.
The relationship between macrophages and the terminal C5b-9 complement complexes was investigated in human arteries affected with atherosclerosis by using monoclonal antibodies and indirect immunoperoxidase, immunogold silver staining, and double-labeling immunohistochemical techniques. Macrophages were found in all the atherosclerotic arteries as immunoreactive deposits with a nucleus, considered as intact cells, or without a nucleus, considered as cell remnants. The double-labeling technique shows C5b-9 deposits partially colocalized on the intact macrophages or on the cell debris of macrophage origin. These data suggest that C5b-9 complement complex may be formed on activated or dying macrophages with subsequent promotion of inflammatory events and progression of the atherosclerotic lesions.
利用单克隆抗体以及间接免疫过氧化物酶、免疫金银染色和双标记免疫组织化学技术，对动脉粥样硬化患者的人类动脉中巨噬细胞与终末C5b-9补体复合物之间的关系进行了研究。在所有动脉粥样硬化动脉中均发现巨噬细胞，表现为有细胞核的免疫反应性沉积物，被视为完整细胞，或无细胞核的免疫反应性沉积物，被视为细胞残余物。双标记技术显示C5b-9沉积物部分共定位于完整巨噬细胞或巨噬细胞来源的细胞碎片上。这些数据表明，C5b-9补体复合物可能在活化或濒死的巨噬细胞上形成，随后促进炎症事件和动脉粥样硬化病变的进展。
