Department of Immunology, Microbiology and Parasitology, University of the Basque Country UPV/EHU, 48940 Leioa, Spain.
Department of Plant Biology and Ecology, University of the Basque Country UPV/EHU, 48940 Leioa, Spain; IKERBASQUE, Basque Foundation for Science, Maria Diaz de Haro 3, 48013 Bilbao, Spain.
Genomics. 2019 Dec;111(6):1547-1556. doi: 10.1016/j.ygeno.2018.10.013. Epub 2018 Nov 10.
Vibrio harveyi is a Gram-negative pathogenic bacterium ubiquitously present in natural aquatic systems. Although environmental adaptability in V. harveyi may be enabled by profound reprogramming of gene expression previously observed during responses to starvation, suboptimal temperatures and other stress factors, the key characteristics of V. harveyi transcripts and operons, such as their boundaries and size as well as location of small RNA genes, remain largely unknown. To reveal the main features of the V. harveyi transcriptome, total RNA of this organism was analyzed by differential RNA sequencing (dRNA-seq). Analysis of the dRNA-seq data made it possible to define the primary transcriptome of V. harveyi along with cis-acting regulatory elements (riboswitches and leader sequences) and new genes. The latter encode a number of putative polypeptides and new phylogenetically conserved antisense RNAs potentially involved in the post-transcriptional control of gene expression.
哈维弧菌是一种革兰氏阴性的致病性细菌，普遍存在于自然水生系统中。尽管哈维弧菌可能通过在应对饥饿、不适宜温度和其他应激因素时先前观察到的基因表达的深刻重编程来实现环境适应性，但哈维弧菌转录物和操纵子的关键特征，如它们的边界和大小以及小 RNA 基因的位置，在很大程度上仍然未知。为了揭示哈维弧菌转录组的主要特征，通过差异 RNA 测序（dRNA-seq）对该生物体的总 RNA 进行了分析。对 dRNA-seq 数据的分析使得能够定义哈维弧菌的初级转录组，以及顺式作用调节元件（核酶和前导序列）和新基因。后者编码许多假定的多肽和新的系统发育保守的反义 RNA，它们可能参与基因表达的转录后调控。
