MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, China.
MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, State Key Laboratory of Urban Water Resource and Environment, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, China.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:524-533. doi: 10.1016/j.msec.2018.09.061. Epub 2018 Oct 1.
Catalytic reduction of toxic 4‑nitrophenol to 4‑aminophenol and dye wastewater treatment over rapid, convenient gold nanoparticles or laccase decorated hybrid vesicles catalysts has attracted much attention. In current work, a stable building block was designed with inorganic gold nanoparticles and nano-conjugates; and a hybrid giant vesicles (AuNPs@vesicles) was self-assembled by using Pickering emulsion method. The vesicles were characterized by SEM, TEM, UV-vis and DLS measurements. The results showed that a temperature-responsive multifunctional building block based on BSA-PNIPAAm and gold nanoparticles was obtained. DLS results also indicated that the length of chains on the surface of AuNPs could change shorter with increasing of temperature (>32 °C) and also obtain an average diameter to ~190 nm. A substrate-rich (high concentration of 4‑nitrophenol) microenvironment can be created around AuNPs, which can dramatically accelerate the interfacial AuNPs-catalyzed reactions. The AuNPs@vesicles as catalyst in the presence of freshly prepared NaBH has excellent catalytic performance for reduction of 4‑nitrophenol (almost 100%). After laccase was capsulated into AuNPs@vesicles, the obtained active hybrid laccase⊂AuNPs@vesicles demonstrated high catalytic decolouration efficiency (>98.5%, nearly 2.3 times higher than that of free laccase) and excellent reusability. The possible mechanisms of reduction of 4‑nitrophenol and dye decolouration was proposed. These novel giant vesicles could provide some new opportunities in wastewater treatment, bottom-up synthetic biology, bioinspired microstorage/microreactor and drug/gene delivery.
在快速、便捷的金纳米粒子或漆酶修饰的混合囊泡催化剂的作用下，将有毒的 4-硝基苯酚催化还原为 4-氨基酚和处理染料废水引起了广泛关注。在当前的工作中，设计了一种由无机金纳米粒子和纳米缀合物组成的稳定构建块；并通过 Pickering 乳液法自组装了一种混合巨型囊泡（AuNPs@vesicles）。通过 SEM、TEM、UV-vis 和 DLS 测量对囊泡进行了表征。结果表明，得到了一种基于 BSA-PNIPAAm 和金纳米粒子的温度响应多功能构建块。DLS 结果还表明，AuNPs 表面链的长度可以随着温度的升高（>32°C）而变短，并且可以获得约 190nm 的平均直径。可以在 AuNPs 周围创建一个底物丰富（高浓度 4-硝基苯酚）的微环境，这可以极大地加速界面 AuNPs 催化反应。在新鲜制备的 NaBH 存在下，作为催化剂的 AuNPs@vesicles 对 4-硝基苯酚的还原具有优异的催化性能（几乎 100%）。将漆酶封装到 AuNPs@vesicles 中后，得到的活性混合漆酶⊂AuNPs@vesicles 表现出高的催化脱色效率（>98.5%，比游离漆酶高近 2.3 倍）和良好的可重复使用性。提出了还原 4-硝基苯酚和染料脱色的可能机制。这些新型的巨型囊泡在废水处理、自下而上的合成生物学、仿生微储存/微反应器和药物/基因传递等方面提供了一些新的机会。
