Sakai A, Shimizu Y, Hishinuma F
Laboratory of Molecular Genetics, Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Genetics. 1988 Jul;119(3):499-506. doi: 10.1093/genetics/119.3.499.
We have isolated mutants responsible for an oversecretion phenotype in Saccharomyces cerevisiae, using a promoter of SUC2 and the gene coding for alpha-amylase from mouse as a marker of secretion. These mutations defined two complementation groups, designated as ose1 (over secretion) and rgr1 (resistant to glucose repression). The ose1 mutant produced an oversecretion of amylase by 12- to 15-fold under derepressing conditions; however, the amylase mRNA was present at nearly the same amount as it was in the parent cells. No expression of the amylase gene was detected under repressing conditions. The rgr1 mutant oversecreted amylase by 11- to 13-fold under repressing conditions by 15- to 18-fold under derepressing conditions. The rgr1 mutant showed pleiotropic effects on the following cellular functions: (1) resistance to glucose repression, (2) temperature-sensitive lethality, (3) sporulation deficieny in homozygous diploid cells, and (4) abnormal cell morphology. The rgr1 mutation was not allelic with ssn6 and cyc9, and failed to suppress snf1.
我们利用酿酒酵母中SUC2的启动子和小鼠α-淀粉酶的编码基因作为分泌标记，分离出了导致酿酒酵母分泌过量表型的突变体。这些突变定义了两个互补群，分别命名为ose1（过量分泌）和rgr1（对葡萄糖阻遏有抗性）。ose1突变体在去阻遏条件下淀粉酶分泌量增加12至15倍；然而，淀粉酶mRNA的含量与亲代细胞中几乎相同。在阻遏条件下未检测到淀粉酶基因的表达。rgr1突变体在阻遏条件下淀粉酶分泌量增加11至13倍，在去阻遏条件下增加15至18倍。rgr1突变体对以下细胞功能表现出多效性影响：（1）对葡萄糖阻遏的抗性，（2）温度敏感致死性，（3）纯合二倍体细胞的孢子形成缺陷，以及（4）异常的细胞形态。rgr1突变与ssn6和cyc9不等位，并且不能抑制snf1。
