Department of Dermatology, Xi'an No. 3 Hospital, Xi'an, China.
Department of Dermatology, The Eighth Hospital of Xi'an, Xi'an, China.
J Cell Biochem. 2019 Apr;120(4):5417-5423. doi: 10.1002/jcb.27820. Epub 2018 Nov 13.
Long noncoding RNA (lncRNA) FOXD2 adjacent opposite strand RNA 1 (FOXD2-AS1) has been shown to be dysregulated in several types of human cancer. However, the role of FOXD2-AS1 in cutaneous melanoma was still unclear. In our study, FOXD2-AS1 expression has been found to be upregulated in cutaneous melanoma tissue specimens and cell lines compared with that in normal tissue specimens and normal human epidermal melanocyte, respectively. Furthermore, high expression of FOXD2-AS1 was obviously correlated with deep Breslow thickness, present ulceration, high Clark level and distant metastasis in cutaneous melanoma patients. However, there were no statistical associations between FOXD2-AS1 expression and cutaneous melanoma patients' disease-free survival and overall survival. The results of loss-of-function study showed that inhibition of FOXD2-AS1 suppresses cutaneous melanoma cell proliferation, migration and invasion through regulating phospho-Akt expression. In conclusion, FOXD2-AS1 is associated with clinical progression in cutaneous melanoma patients, and functions as oncogenic lncRNA in cutaneous melanoma cells.
长链非编码 RNA（lncRNA）叉头框转录因子 D2 反义 RNA1（FOXD2-AS1）在多种人类癌症中已被证明失调。然而，FOXD2-AS1 在皮肤黑色素瘤中的作用仍不清楚。在我们的研究中，与正常组织标本和正常人表皮黑素细胞相比，FOXD2-AS1 在皮肤黑色素瘤组织标本和细胞系中的表达均上调。此外，FOXD2-AS1 的高表达与皮肤黑色素瘤患者的深 Breslow 厚度、溃疡存在、高 Clark 分级和远处转移明显相关。然而，FOXD2-AS1 的表达与皮肤黑色素瘤患者的无病生存期和总生存期之间没有统计学关联。功能丧失研究的结果表明，抑制 FOXD2-AS1 通过调节磷酸化 Akt 的表达抑制皮肤黑色素瘤细胞的增殖、迁移和侵袭。总之，FOXD2-AS1 与皮肤黑色素瘤患者的临床进展有关，在皮肤黑色素瘤细胞中作为致癌 lncRNA 发挥作用。
